| Strangle is a highly contagious disease of horse caused by Streptococcus equi subsp. equi.Strangle is characterized by fever and abscess formation in the lymph nodes of the head and the neck. The disease occurs worldwide and causes heavy economic losses in terms of the cost of treatment. Streptococcus equi subsp. equi belongs to Lancefield group C, this bacteria has a variety of surface proteins which were important virulence factors and can stimulate the body to produce an immune response.This study investigated the immunogenicity and immune protection of surface proteins of Streptococcus equi strains XZ1 isolated in Xinjiang.To study the immunogenicity of Streptococcus equi subsp. equi, we extracted the bacterial protein and detected it by SDS-PAGE. Then the extracted protein was used to react with rabbit serum that come from rabbit immunized with Streptococcus equi subsp. equi and then the protein was reacted with serum from recovered horse using western-blot. Our result indicated that the protein has good immune reactivity with these antiboty.In order to detect immnue efficacy of the bacterial protein extracted from Streptococcus equi subsp. equi XZ1 strain isolated in Xinjiang. The extracted bacterial protein was used to immunize mouse and the serum was collected to examine antibodies after immunization.Then the immunized mouse was challenged with this bacteria strain. Our result indicated that the outer protein had better immunogenicity on mouse and the protection rate of the bacterial protein was 67%.To further verify the immune effects of the extracted bacterial proteins in horses, the proteins was used to immune horse. Our results showed that compared with the control group the bacterial protein can induce the horse to produce specific antibodies and significantly stimulate cell immunization compared with control group(P<0.01). This result lay the foundation for further development of the prevention and control measure of strangle. |
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