Functional Analysis Of Leucine-rich Repeat Receptor Kinase LRK2 In Rice(Oryza Sativa L.)

Rice yield traits belong to the typical quantitative characters, genetically controlled by multiple quantitative trait loci (QTLs).They are mainly composed of effective panicles per plant, grains per panicle and grain weight. Rice is important monocot model plant, its yield traits mechanism is very complex. The quantitative trait locus (QTL) has become an important area in today’s genetic research. Previous research has fine mapping that high yield QTL candidate genes for leucine-rich repeat receptor protein kinase gene cluster LRK. The cluster in dongxiang wild rice contains eight genes (LRK1-8). Our research found overexpression LRK2 gene significantly improved indica nipponbare effective panicles per plant. Subcellular localization experiments showed LRK2 protein localizied on the plasma membrane. Gus staining result show that lecine-rich repeat receptor protein kinase LRK2 gene, express in rice root, tiller buds, node and flowers.The rice resistance is in the process of plant growth and environmental adaptation, the rice shows the defense capability to various stress factors (drought resistance, salt resistance, the resistant to diseases and insects) The rice is the most important crops in the world, it has a big demand for the fresh water resources, so the drought is the main stress factor affect rice yield, so we study the mechanism to drought stress that is a effective method to reduce water shortages on the rice yield loss is of great importance.In order to further explore the rice LRK2 gene function, this article is mainly introduce some treatment to LRK2 overexprssion lines, explore its role under adversity, then use the method of real-time quantitative PCR explore LRK2 response to drought stress, study the mechanism of drought resistance. That’s a basis to fully disclose the roles receptor protein kinase in the growth and development,accept the signals produced by drought stress and active the downstream signaling pathways, triggered a series of process of signal transduction. Physiological experiments show that transgenic plants reaction to drought stress stimuli sensitive.