The Oil Bodv Development Regularitie In Tung Tree (Vernicia Fordii) Seed And FADX、PDAT1 Gene Clone And Expression

The tung tree (Vernicia fordii), a member of the Euphorbiaceae family, is an important industrial oilseed plant, originating in China and it is one of China’s four major woody species. The oil, named tung oil, accumulates in tung seeds with 80% α-eleostearic acid in total oil content. Tung oil has important economic values and now widely used in the production of paints, plasticizers, high-quality printing, resins, and medicine. FADX is a key gene involved in the accumulation of Eleostearic Acid and seed-specific expression in tung tree. FADX encoded a fatty acid binding enzyme, which can catalyze linoleic acid to a-eleostearic. PDAT encoded phospholipid diacylglycerol acyltransferase (PDAT), PDAT enzymes contributed to unusual fatty acid modified on PC(phosphatidylcholine) accumulation into TAG. In this paper, on one hand, we detected development of oil body in tung seed during different period by electron and optical microscopy observation to explore the development law of oil body; Besides, we analyzed the total oil content and fatty acid composition in tung seed during different period to explore the relationship between oil content and oil body and the critical period of Eleostearic Acid formation; These work can make a contribution to detection of key genes involved in the accumulation of Eleostearic Acid. On the other hand, we cloned FADX which were over expressed in Arabidopsis Col-0 and mutant dagtl. While, we cloned PDAT1 and over expressed PDAT1 in Arabidopsis fadx/fadx3/fael. These work made a foundation for preliminary exploring the Eleostearic Acid accumulation pathway and the function of FADX and PDATl. The main results are as follows:1. The critical period for oil body development is the 88-130 d after pollination; the critical period for tung oil synthesis is 81-119 d pollination. The total oil content is positively related to the oil body development. The key period for Eleostearic Acid formation is 74-98 d pollination, with the tendency of "S".2. The FADX, PDAT1 gene were cloned in tung seed, FADX cDNA with1221 bp.PDAT1 with 2013 bp, a ORF from30 bp to 2012 bp,coding 661 aa,2 trasembrane areas. FADX, PDAT1 expression vector was successfully constructed, and the infection of Arabidopsis plants were obtained.