Effect Of Precipitation Variation On Distribution Pattern Of Soil Fungi Construct And Function Diversity In Broad-leaved Korean Pine Mixed Forest

The zonal climax vegetation in northeastern China is broad-leaved Korean pine mixed forest; this type of vegetation plays a significant ecological role in the region. Changbai Mountain is an ideal area to study the effects, either positive or negative, of variation in precipitation on temperate forests. In this study,we measured soil catalase, urease, invertase and acid phosphatase activity of seasonal changes in broad-leaf Korean pine mixed forest in Changbai Mountain and based on terminal restriction length polymorphism and Biolog,we also analyzed soil fungal diversity of spatial heterogeneity in the plot which were designed to increase precipitation by 30%[increased(+)] or decrease precipitation by 30%[decreased (-)].The main conclusions are as follows:1. Precipitation did not have a significant impact on the activity of soil enzymes. For the different response of soil enzyme activities inconsistent changes.Four activity changes with the seasons change significantly, catalase activity of the highest in June, invertase and acid phosphatase activity of the highest in August and urease activity highest in September, this enzyme and its function is closely related to seasonal changes. Between soil properties and soil microbial activity and soil physical and chemical have significant or very significant positive correlation. There are highly significant positive correlation between the enzyme and the enzyme.2. Comparing the community and functional diversity of soil microbial under different precipitation and different spatial,we found that soil microbial diversity on two levels are more sensitive to spatial position, the performance of rhizosphere soil> 0-5cm topsoil> 5-10cm topsoil. Increasing and decreasing of precipitation could increase community diversity of soil fungi, but not significant. While the dominant populations vary. The abundance of T-RFs longer than 500 bp increased with precipitation in topsoil, and T-RFs which is in response to the precipitation in rhizosphere were 380,455 and 487 bp, and response pattern were more complex than the topsoil. Rainfall for soil microbial diversity index is not obvious.3.At present study,The changes of AWCD value response to precipitation in the initial culture(48h) and the end of the culture (168h).In the initial, presentation of precipitation 30 percent reduction plots> precipitation increased by 30%> for still, but in the end the value have no regularity. Precipitation changes 30% did not have a significant impact on the microbial diversity.Carbon type utilization of soil microbial of Korean pine broadleaf forest were amino, carbohydrates and multi-cluster. Carbohydrates and amino acids are the most sensitive carbon, especially D-N-acetyl glucosamine, D-cellobiose and a-D-lactose.