Proteomics And Metabolomics Analysis Of Wheat Stripe Rust Resistance Near Isogenic Line Taichung29~*6/Yr10

Wheat is the largest food crop in the world and is the second largest food crop after rice in our country, it’s production accounts for about22%of the country’s total grain output. The annual consumption of100million tons or more, while other expenses such as for processing food, seed, and wear and tear of about12million tons. Because of the growing population, the demand for wheat is also rapid growth. Therefore, maintaining a steady growth in wheat production is of great significance to keep our food security of country and the world. The yield loss is huge caused by pests and diseases. Wheat stripe rust has been a major disease of wheat, and a serious threat to our wheat production and food security. The use of resistant varieties is the most viable method to prevent the wheat strip rust. In this study, we used the wheat stripe rust resistance gene YrlO near isogenic line(NIL) Taichung29*6/Yr10with proteomic and metabolomics method aiming at finding the proteins and metabolites that associated with resistance to stripe rust. So as to lay the theoretical foundation of the prevention wheat stripe rust, and the cultivation of new varieties. The results are as follows:Firstly, we established and improved the system for separate the total protein in wheat leaves. There are1275±34and1303±38particular protein spots were separated by2-DE in T29and TYr10leaves. In these two samples, there are967protein spots were matched by Image Master2D Platinum6.01、 We also found5specific-expressed proteins in TYr10.Secondly, the protein expression of a wheat NIL pair, Taichung29*6/Yr10and Taichung29, have been compared with label-free quantitative proteomic approach based on liquid chromatography tandem Q Exactive hybrid quadrupole-Orbitrap mass spectrometer. By MASCOT database search, a total of2257proteins were identified from both of the samples, and among of them1549proteins were accurately quantitated and102differentially expressed proteins (>2fold) were detected. Gene ontology (GO) analysis showed that these differential proteins were mainly localized to cell matrix and organelles such as ribosome, and mainly involved in binding and catalytic functions. Among of102proteins which mainly take part in metabolic, cellular and stress responses processes, Superoxide dismutase, Methionine aminopeptidase, Lysosomal-β-glucosidase, and Ferritinmay play important roles in Taichung29*6/Yr10resistance to stripe rust.Thirdly, An analytical strategy for the secondary metabolites of wheat leaves was developed based on gas chromatography mass spectrometry (GC/MS),we established and improved the system for separate the secondary metabolites in wheat leaves.143and136metabolites were detected in T29and TYr10leaves, then121of which were structurally identified by searching in the NIST standard mass spectral library, covering carbohydrates, organic acids, steroids and so on which showed a broad coverage of metabolite data. According to compered the peak area, I also found21metabolites that obvious differences in content.Among the differences in proteins and metabolites, SOD and Vitamin E both are important free radical scavengers in plants. Increase of them in TYr10explain that radical scavenging have been improved either in the protein level or in the metabolite levels, therefore, the performance of resistant to stripe rust of TYr10likely to have a significant relationship with its anti-oxidative capacity.