| Brassica napus was formed-7500years ago by hybridization between B. rapa and B. Oleracea, and become allopolyploid afterchromosome doubling. In china and the world, the rape is the second largest oil crops, it plays an important role in the Chinese nutrition supply. With the development of sequencing technologies, transcriptome sequencing is widely used in all research fields of life science. Currently, genome sequence of Brassica napus was published, providing a good foundation for uncovering genetic developmental mechanism of Brassica napusoil content, In this study, the pod transcriptomes of Chinese variety Gaoyou and Germany variety Sollux were sequenced by the second generation sequencing platform Illumina Hiseq2000at three different growth stages. Differential expression analysis of genes based on RNA-seq data was implemented, and the expression genes were annotated by GO (Gene Ontology) and pathway information. Then we conducted integrated analysis of the gene expression information, the lipid-related genes of Arabidopsis and the oil content-related QTLs of Brassica napus for mining and analyzing candidate functional genes associated with oil content. Main results are as follows:1,465and2,114differentially expressed genes (DEGs) were identified, respectively, between two varieties at the same stages and between different growth stages of each variety;2, Eight differentially expressed genes between two varieties were identified that homologous with Arabidopsislipid-related genes. The expression pattern of differentially expressed Arabidopsis lipid-related genes were analyzed, results indicate that most of these genes were up-regulated with pod development;3,33differentially expressed genes between two varieties were identified in the QTL regions, of which, one was found to be homologous to Arabidopsis thaliana lipid-related genes and could be regarded as a candidate gene with potential value in genetic improvement of oil content. |
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