Simulation Of Space Mutation To Sift Excellent Strains Of Pleurotus Eryngii

In recent years, Pleurotus eryngii is the fastest growing market of integrating food medicine, health care function for a kind of edible fungi. In this study, we option8of high quality Pleurotus eryngii strain to the mushroom experiment, and collecting sterile single spore and diluted into suspension. By the "Edible and medicinal fungi strains of simulation of space mutation breeding apparatus" to induced the spores. The new pairs compare parent are get the new high quality Pleurotus eryngii strain, through antagonism experiment, ISSR molecular identification, Fruiting body comparison and composition comparison experiment. The experimental results are as follows.Through the parents of the mushroom experiment, we collected a total of five mutagenesis spore suspension, and parents of the same concentration of spore germination rate before and after mutagenesis, mutagenic effect is the most ideal is15spores, the fatality rate is95%,10,21,25, is not in conformity with the mutation related conditions, selection.20and fatality rate of90%.No.10,25mutagenesis fatality rate was100%, the spore germination rate is zero, no research significance, and21,22, spore under the condition of the same mutation, failed to achieve mutagenic effect.Through the15varieties of spore single spore matching after mutagenesis, got23pairs of two affinity. By microscopy, eliminated four groups of mononuclear mycelia, get19pairs of dual-core hyphae. It through the experiment of antagonism, with parents pick obvious antagonism phenomenon occurs with parents with11pairs new combination.11equipping mycelium character comparison experiments of combination and15parent, eliminated the T3, T4, T6, T8, Ti1the combination of comprehensive performance is poor in5groups of parents, the T1, T2, T5, T7has, T9, and T10the six excellent in comprehensive characteristic variation group.All strains by ISSR analysis, divided into four categories, only the first I apricot15, parents for the first II mutant strain T1, T10, for the first III mutant strain T2, T5and T7has, for the first IV mutant strain T9. Mutant strain has kept most of the genetic material, but the T1, T2, T5, T7has, T9, and T10and the parents, there is a certain genetic variation between six mutant strain and the variation degree and the position of each different.According to the mushroom makes a comprehensive comparison between the experimental and get three kinds of high quality Pleurotus eryngii strains. T5strain has a strong resilience, can not only a certain amount of resistance to high temperature, and the ability to resist infectious microbe is very prominent. T2strain of the agronomic traits of the indicators have reached the market standard, and the biological conversion rate higher than the parent, reaching75.36%. For comprehensive consideration the three main nutrients, The nutrition content of T7strain is higher than the parent and other mutant strains.