Studies The Structures On Pomacea Canaliculata Of Morphology And Flora For Digestive System

Objective:Pomacea canaliculata, which was included in the first batch of China’s invasion of alien species, cause great damage on China’s agricultural production. But less studies on the basis of Pomacea canaliculata,so in our study, studied the morphology and the bacterial population structure of the digestive system of Pomacea canaliculatas, and provide a theoretical basis for the study of Pomacea canaliculata.Methods:Studied the morphology of the digestive system of Pomacea canaliculatas by anatomy the digestive system and making paraffin section.And the bacterial population structure and diversity of gastrointestinal microbiota associated with the male and female Pomacea canaliculatas were analyzed by denaturing gradient gel electrophoresis (DGGE), and the main bands were recovered, cloned and sequencing. Intestinal bacteria from the Pomacea canaliculatas were isolated and cultured, identification of bacteria through physiological and biochemical identification and16SrDNA sequence identification.Results:1The morphology of the digestive system of Pomacea canaliculatas:There are a large number of secretory cells in the digestive gland of Pomacea canaliculatas, and Secretion exuberant. Pomacea canaliculatas’digestive tract has developed relatively thick muscular layer and there is cartilaginous gastric shield in the stomach, this is the basis of rapid physical and chemical digestion of Pomacea canaliculata.2The bacterial population structure of the digestive system of Pomacea canaliculatas:Flora bacteria is in much the same way between in stomach and in intestine of male and female Pomacea canaliculata. Sequencing results show these bacteria belonged to the genus Aeromonas, Shewanella, Serratia marcescens, Enterobacte^Rainbow trout intestinal bacterium, uncultured bacteria isolated by DGGE, non-culturable bacterial clones.3Isolation and culture of intestinal bacteria:Two strains of intestinal bacteria from the Pomacea canaliculatas were isolated and cultured, through physiological and biochemical identification and16SrDNA sequence identification, the results indicate that these two bacteria were Enterobacter aerogenes and corruption Shewanella.Conclusion:The bacterial population structure of the digestive system of Pomacea canaliculatas was first analyzed, and proof that the flora bacteria is in much the same way between in stomach and in intestine of male and female Pomacea, and further isolated and cultured the bacteria in the gut, the above research work provide a theoretical basis for Pomacea canaliculatas.