Effects Of Fluoride On Testicular Immune Exemption And Its Meachanism Study In Male Mice

[Objective] To study the influence of of different concentrations of sodium fluoride on the close connection between testicular sertoli cells in mice, and conduct the toxicity test of sertoli cells in vitro, to explore Effects of Fluoride on sertoli cells, in order to lay the foundation for further exploring the damage mechanism of fluoride on male reproductive function.[Method] 100 healthy Kunming male mice(8 weeks old) were randomly divided into 4 groups and exposed to 25,50, 100mg NaF/L in their drinking water for 8 weeks. During the whole test, record regularly drinking water volume and weight of the experimental mice. At the end of the exposure periods, calculating how much fluoride the mice take every day, measuring the fluorine content in femurs, and Observed the testicular tight connection under electron microscope.sertoli cells Isolated and cultured of male mice(15-20days) were randomly divided into 4 groups and exposed to10-5,10-4,10-3 mol NaF/L in the culture medium for 48h. At the end of the exposure periods, Part of SCs were transplanted to renal capsule, peripheral lymphocyte apoptosis rate of graft was detected by TUNEL after which, the other part was used for protein and gene detection about immue privilege.[Results] 1. After 8 weeks exposure, there were no significant difference in body weight and was observed between NaF treatment groups and control groups. In the femoral fluorine content assessment,25 mg NaF/L treatment group,50mg NaF/L treatment group and 100mg NaF/L treatment group showed significant difference compared with control group(P<0.05).2. Electron Microscopy photos showed that high does fluoride would demage the structure of testis tight connection.3. In the SABC test, With the increase of concentration of sodium fluoride, the number of SCs in renal subcapsular declined. About lymphocyte apoptosis rate, 100mg NaF/L treatment group showed a significant increase compared with control group.4. According to the results of QRT-PCR, level of TGF-β1 and FasL mRNA was decreased in NaF groups. Level of TGF-β1 mRNA was decreased, and FasL mRNA was decreased significantly in100mg NaF/L treatment groups. Whereas, The other mRNA of target genes detected no statistical difference.5. According to the results of western blot, expression of TGF-β1 and FasL was decreased in NaF groups. Expression of TGF-β1 was decreased, and FasL was decreased significantly in100mg NaF/L treatment groups. Whereas, The other target protein expression detected no statistical difference.[Conclusion] Taken together, our study showed that a certain concentration of fluoride would damage structure of the testicular tight connection and function of immue privilege of SCs. The toxicity mechanism may include the decrease of mRNA and protein expression of TGF-β1 and FasL.