Regulation Of Anthocyanin Biosynthesis In Tomato By MiR828

Anthocyanin is a kind of water-soluble natural edible pigment, it not only plays a role in plant growth and development, but also has the effect of health care. Tomato is one of the largest fruit and vegetable consumption in the world, and also commonly used to model plant in molecular biology research, But the content of anthocyanin was very low in common tomato fruits. Therefore, in recent years, the content of anthocyanin in tomato has become an important research field of tomato quality improvement. MiRNAs are a class of about 20-24 nt single-stranded non-coding small RNA, primarily through the post-transcriptional level affects the expression of target genes and controlling the growth of organnisms。 The discovery of miRNAs mechanism of anthocyanin synthesis provided a new way in the post-transcriptional level. MiR828 was known as a new kind of miRNA in recent years by deep sequencing, and not too detailed study of its function.In order to identified the target genes of tomato miR828 and miR828 role in anthocyanin synthesis. In this study, By the bioinformatics software and qRT-PCR method to predicted and verified the two target genes were:anthocyanin synthesis related MYB family of transcription factors Sly-myb-like (SGN-U320618) and an expression protein SGN-U317526. By the qRT-PCR method to detected T1 generation miR828 overexpression transgenic tomato on target gene mRNA levels and changed in anthocyanin synthase gene and anthocyanin content, to explored the role of miR828 regulation of anthocyanin synthesis and miR828 expression of anthocyanin synthesis in phosphorus deficiency stress.The results showed that:(1) Sly-myb-like (SGN-U320618) and SGN-U317526 were miR828 target genes in tomato. (2) By qRT-PCR analysed of miR828 expression in different tissues of wild tomato, its expression levels were very low in almost all tissues. Which is relatively high, expressed in roots, stems, buds and green fruit.but is the least in the mature fruit. (3) The target gene and anthocyanin synthesis related enzyme gene expression were in different degrees of reduction, and the anthocyanin was decreased significantly in transgenic tomato plants. (4) P deficiency stress, miR828 played a negative regulation of anthocyanin synthesis related gene expression by targeting Sly-myb-like and SGN-U317526, thereby negatively regulated the biosynthesis of tomato plants in anthocyanins.