Study On The Biochemical Mechanisms Of Picea Schrenkiana Autotoxicity In Seed Germination And Seedling Growth

In the limited time without fire disturbance in forest restoration, autotoxicity is one of the main factors leading to barrier of population natural regeneration of Picea schrenkiana Fisch.et Mey., but it is unclear to reveal what the biochemical mechanisms of P. schrenkiana autotoxicity is. This paper on the basis of known autotoxicity materials DHAP of P. schrenkiana, on the condition of artificial intelligence environment simulation, oriented to biometrics, by means of detection technology of modern instrumental analysis and separation, studies the effect of apparent biological characteristics and activities of antioxidant enzymes of P. schrenkiana during seed germination and seedling growth under the concentration of autotoxicity substances DHAP, explains response of antioxidant enzymes to changes of toxic substances DHAP concentration during seed germination and seedling growth, reveals the biochemical mechanisms of P. schrenkiana autotoxicity during seeds germination and seedlings growth. The main achievements are as follows:The influence of DHAP treatment on P. schrenkiana in seed germination and seedling growth show "low promotion high suppression" phenomenon, and the concentration of 1.0 mmol/L show the strongest promote effect, concentration of 3.0 mmol/L begin to inhibit significantly, the higher the concentration, the stronger the inhibition.When DHAP concentration is higher than the critical concentration of autotoxicity, it will begin to damage cells, so cells can’t improve the activities of SOD, POD, CAT, APX, GR to resist DHAP stress, the activities of SOD, POD, CAT, APX, GR significantly reduce, so delay seeds germinate, reduce the seed germination potential and germination rate finally, lead to seedling plumules and radicles elongation slow, fresh weight decrease.DHAP concentration influence the activities of SOD, POD, CAT, APX and GR during seed germination and seedling growth of P. schrenkiana, then the change of the activities of SOD, POD, CAT, APX, GR regulate seed germination and seedling growth, and cause many apparent biological characteristics changes of P. schrenkiana during seed germination and seedling growth.Under the effect of the autotoxicity of DHAP, SOD, POD, CAT, APX and GR, the five kinds of enzyme coordinate to remove the ROS produced in the body and reduce the damage to cells, resisting DHAP stress, in order to ensure the normal growth and development of P. schrenkiana.