| In this paper, we used I. ensata as the research object which was wild species with high ornamental value, and used its pollen and seeds as material to sduty germplasm preservation technology, and also used seed embryos as explants to establish propagation system. The results could provide reference for the germplasm conservation, sustainable use and reproduction of I, ensata and other plants of Iris. The main findings are as follows:1. We determined the pollen viability of I. ensata with the way of vitro germination and the optimal formula of vitro germination medium was sucrose(150g·L-1)+H3BO3(200mg·L-1)+CaCl2(150mg·L-1)+Fe2(SO4)3(50mg·L-1)+KNO3(50mg·L-1)+MgSO4·7H2O(100mg·L-1).2. We took two ways TTC staining and germination in vitro to determine the pollen viability in different florescence, the results showede that they had high pollen viability from early flowering to flowering.3. It was about 20% witch was the optimum moisture for cryopreservation of I, ensata pollen, the thawing methods were both bath and tap water, at that time the pollen viability rised from 56.04% before cryopreservation to 60.53% after cryopreservation.4.The anther of I. ensata could also be cryopreserved. But the germination rate after cryopreservation decreased compared with the rate before cryopreservation, the pollen viability was 63.09% witch was measured with the method of TTC staining, and it was 54.11% when was measured with the method of germination in vitro.5. We did the drying process for I. ensata seeds after harvested, it wasn’t fatal injuries for I. ensata seeds when the moisture dropped from 9.8% at the beginning to 1.6%, and their germination rate and germination potential could also reach 88.57% and 84.00%.6. It was 3.8% witch was the optimum moisture of I, ensata seeds for cryopreservation, and the way for I. ensata seeds to thaw after cryopreservation was water thawing, with this method the germination rate and germination potential improved respectively about 12.00% and 14.00%. compared with the the germination rate and germination potential in the initial moisture content before cryopreservation, they were 93.89% and 87.11%.7. In the research of propagation technology the seeds of I, ensata must be soaked for 10 minutes in the right amount and diluted detergent before seed embryos were stripped from these seeds, and then put them in the clean bench, disinfecht 1 minutes with 75% alcohol, disinfecht 30minutes with 40% NaCIO, rinsed with sterile water 5 to 6 times, each time for 1 minutes, finally the contamination rate of Iris ensata seeds dropped to 5.67%.8. Seed embryos are good explants to induce cluster buds, it is MS+ 6-BA1.0 mg·L-1+NAA0.6 mg·L-1 +KT2.0mg·L-1 that is the optimal media formulation for inducing and proliferation. The multiplication factor was up to 9.61 when they were cultured for 60 days to statistic.9. The optimum medium for rooting is MS+NAA0.5mg·L-1 and the rooting rate is 100%; the survival rate of these plantlet reached to 83% after they transplanted to matrix that the rate of garden soil and vermiculite was 1:1, and they were cultured 20 days in the rooting medium. |
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