Gene Discovery And Expression Analysis Of PDR Transporters Based On Blueberry Transcriptome Analysis

Blueberry(Vaccinium spp.) is one of the best development prospects fruits trees. It not only has unique nutritional health benefits, but also has the effect of treatment and prevention of cancer and other diseases. Abundant secondary metabolism products in blueberry, especially anthocyanins, play an important role on them. Plant PDR transporters play an important role in the transporter and accumulation process of secondary metabolites. So learning PDR transporter genes of blueberry has an important significance for futher explaining the molecular mechanism of secondary metabolites formation in blueberry, explaining plant defense response mechanism and breeding new varieties of blueberry. However, blueberry tissue-specific transcriptome and genomic data in public databases are not sufficient for an understanding of the molecular mechanisms associated with secondary metabolites. The bioinformatics was used to identify blueberry PDR transporters based on the transcriptome sequencing library of blueberry fruit. And the real-time quantitative PCR was performed to analysis the relative expression levels of PDR genes in different organs and tissues of blueberry. The main results are as follows:1. 165 ABC transporters genes were selected based on the transcriptome sequencing library of blueberry fruit. And according to their annotation, most of the 165 ABC transporter genes were divided into ABCA subfamily(14 genes), ABCB subfamily(32 genes), ABCC subfamily(30 genes), ABCD subfamily(2 genes), ABCF subfamily(8 genes) and ABCG subfamily(57 genes). The rest 22 of them were not be found the subfamily their belonging to.2. ABCG subfamily, which includes 30 PDR transporter genes and 21 WBC transporter genes, is the largest one of ABC transporter in blueberry. There were different expression between pericarp and sarcocarp gene bank of blueberry. The 21 of PDR transporter genes were up-regulated genes in the skins of blueberry, in which there were 13 up-regulated genes annotated to participate in the defense mechanism in the COG databases.3. The q RT-PCR results demonstrated that 21 PDR transporter genes were expressed in all of the organs and tissues of blueberry and shown tissue specificity. Among them, 16 genes had the highest expression levels in the roots.