Differentially Expressed Genes In Adipose Tissue Research RETN, CAV1, PLA2G16 Altay Sheep Tail Fat Deposition And Metabolic Relationships

Altay sheep is a breed of fatty varieties sheep. They achieve the maintenance of the body’s normal metabolism and temperature mainly through the tail fat deposition in metabolism dynamic of harsh environment, which are ideal model for studying uropygial deposition and Metabolism. Resistin(RETN), caveolin-1(CAV1) and adipose-specific phospholipase A2(PLA2G16) are key regulators of fatty cells, which play an important role in cell differentiation and multiplication etc. Recent studies have showed that the three factors included RETN, CAV1, PLA2G16 involved in the process of deposition and metabolic of animal tallow. To further confirm the impact of the three factors above on the deposition and metabolic characters of tail fat of sheep, this research was conducted by using PCR technique to clone RETN, CAV1, PLA2G16 genes of sheep and analyzed the bioinformatic of its sequence, which was based on the simulation of Altay sheep fat deposition in the summer season and spring and winter-saving way to end lipid metabolism with less of grass; Uing semiquantitative RT-PCR method to detect the genes expression of RETN, CAV1, PLA2G16 in major tissues of sheep; While taking advantage of real-time PCR technology to detect the differences of gene expressions of RETN, CAV1 and PLA2G16 in Altay sheep’s tail fat between the continuing state of hunger and adequate intake situation. The main results are as follows:1. Constructing experimental model of hunger and non-hunger by simulating its fat deposition in the summer and autumn, spring and winter when the grass-saving way to end the lack of fat metabolism based on Altay sheep.2. Obtaining complete gene sequences of RETN, CAV1 and PLA2G16 by RT-PCR in CDS regions with using Altay sheep tail fat tissue c DNA as a template.3. Semi-quantitative RT-PCR test results showed that, RETN, CAV1, PLA2G16 genes were highly expressed both in Altay sheep adipose tissues(fat gut and tail fat tissue). It means that RETN, CAV1, PLA2G16 genes play an important role in physiological processes in Altay sheep tail fat.4. Applying real-time PCR technology to detect the different expressions of RETN, CAV1, PLA2G16 genes in Altay sheep tail fat tissue under constant state of hunger. The results showed that there was a significant difference in expression level of RETN gene before and after the test in Altay sheep tail fat tissue, and the expression level of RETN in the situation of persistent hunger was significantly higher than that under normal feeding state(P <0.01). On the contrary, the expression level of CAV1 and PLA2G16 gene were significantly higher in normal feed(non-starvation) than that under constant state of hunger in Altay sheep tail fat tissue(P <0.01). These results indicated that RETN, CAV1 and PLA2G16 genes play a role in the process of regulating metabolism in Altay sheep tail fat deposition, which can be used as candidacy functional genes to regulate the processes of growth of mammalian adipose tissue.