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Effect Of Different Treatments Of Bean Plants On Development And Enzyme Activities Of Frankliniella Occidentalis(Pergande)

Posted on:2016-09-02Degree:MasterType:Thesis
Country:ChinaCandidate:F MuFull Text:PDF
GTID:2283330479955616Subject:Agricultural Entomology and Pest Control
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The western flower thrips, Frankliniella occidentalis(Pergande), is one of the most dangerous pests around the world, F. occidentalis has caused serious damage on flowers, vegetables and other economic crops since it was firstly recorded in Guizhou in 2006. In this paper, kidney beans, one of the main planting vegetables in Guizhou, was chosen as the host plant of the F. occidentalis, the impact of induced resistance of kidney bean on growth, activities of detoxification enzyme, protective enzyme, digestive enzyme in 2nd larval of F. occidentalis were asessed by different treatments of damage by thrips’ feeding(DTF), mechanical damage(MD), and chemical inductive substances(methy salicylate, Jasmonic acid). The mehnismes of different exogenous factors to induce resistance of F. occidentalis were discussed, and the resuls will provide a theoretical basis of using induced resistance of plants to control F. occidentalis and thrips management. The results were as follows:1. Effect of different treatments of kidney bean plants on the development and population of F. occidentalisThe different treatments of kidney bean(damage by thrips’ feeding, mechanical damage, methy salicylate, jasmonic acid) on the development and population of F. occidentalis were studied. The results showed that the developmental durations of immature stage of F. occidentalis induced by DTF, exogenous MeSA and JA was significantly prolonged, which was 4.95, 2.87 and 4.72 d longer than that of healthy plant respectively, and the survival rate of F. occidentalis decreased in different treatments. The difference in the development duration of F. occidentalis between healthy and mechanical damaged plants wasn’t significant. The population dynamics of F. occidentalis significantly decreased under different treatments, and JA had the most significant inhibitory effect. Therefore, the kidney bean plants induced by thrips’ feeding and chemical inductive substances had effect on the developmental duration and population dynamics of F. occidentalis, and the effect of induced resistance by JA was bigger than that of MeSA.2. Effect of different treatments of kidney bean plants on activities of detoxification enzyme in F. occidentalisAfter F. occidentalis feeding kidney bean plants by different treatments, The activities of MFO and TChE in 2nd larvae was significantly lower than that of control. In addition, the GSTs activity increased at 24 h after feeding the kidney bean plants by JA induced, however, the activities of GSTs induced by DTF, exogenous MeSA and JA, were higher than control respectively, while GSTs activity in thrips that feeding the mechanical damaged plants wasn’t significant different compared with the control under the different feeding time. The CarE activity was significantly inhibited at 6, 36 h after feeding the kidney bean plant by DTF and JA induced, while activity was significantly lower than the control after feeding the kidney bean plants with the other two treatments. The activities at 6 and 24 h were not significant different compared with the control.3. Effect of different treatments of kidney bean plants on activities of protective enzyme, digestive enzyme in F. occidentalisAfter F. occidentalis feeding kidney bean plants of different treatments, the SOD activity was activated at 6 h compared with the control, the activity of other enzyme were certainly inhibited at 6 and 24 h. With the extention of feeding time, the enzyme activities were higher than that of control at 36 h. The CAT in 2nd larvae was inhibited under the different time, and the enzyme activity in thrips after feeding the kidney bean plants by DTF and JA induced was significantly lower than that of control. The CAT in thrips feeding different treatmens were significantly higher than that of control at 6 h, the enzyme activity in thrips feeding different treatmens decreased gradually with the feeding time extended. The enzyme activity dropped to less than the control at 36 h, especially the enzyme activity of DTF was lowest among the different treatments.Compared with the control, the TPS activity decreased firstly and then increased, however, the activities of TPS induced by three other treatments were all higher than the control. The Chymotrypsin activity in 2nd larvae was lower than that of control under the different feeding time. However, the Chymotrypsin activities induced by DTF, MD and MeSA were higher than that of control at 24 h. The Amylase activities were inhibited in varing degrees and showed significant difference, and the inhibition of DTF and JA were stronger than that of MD and MeSA. The changes of Trehalase activity were complex, and Trehalase activitiesin F. occidentalis feeding bean induced by MeSA and JA were significantly higher than that of control at 6 h. Trehalse activity in F. occidentalis feeding DTF was significantly lower than that of control, no matter feeding time was 24 or 36 h, which indicted that the activities were significantly inhibited.
Keywords/Search Tags:Frankliniella occidentalis, induced resistance, developmental duration, population dynamics, detoxification enzyme, protective enzyme, digestive enzyme
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