Study On The Breeding Biology Of Palaemon Carinicauda

Exopalaemon carinicauda has sone characterics such as long breeding season,fast growth and strong adaptability to environment, etc. In recent years, with the development of the mariculture area to low salinity, morphological and white shrimp farming production was increased rapidly, often mixed with other Marine economic species breeding. For the study of morphological and white shrimp in the regulation mechanism in the process of reproduction, the paper first cloned FAMe T genes, and were measured in three consecutive ovarian development FAMe T gene expression in the morphological and white shrimp. To end white shrimp penaeus chinensis and ridge at the same time in the three consecutive breeding vitellogenin gene with the ovaries tissue section make a comprehensive analysis, this paper expounds the tail white shrimp penaeus chinensis and ridge breeding. The specific research results are as follows:1. The morphological and white shrimp FAMe T gene cloningIn order to study method, acid methyl transferase(FAMe T) on morphological and white shrimp role in the development of ovary, using reverse transcription PCR and c DNA end rapid amplification technology(RACE) cloning get the length of the tail ridge white shrimp FAMe T c DNA sequence(Gen Bank login number: AHB23861), it includes a 76 bp of 5 ’non coding region(untranslated regions, UTR), a 712- bp 3’ noncoding region and a 831- bp open reading frame(open reading frame, ORF). Encoding 276 amino acids, 3 ’UTR with typical plus tail signal AATAAA and Poly real(A) tail. Sequence analysis found that the sequence of amino acid sequence has the characteristics of FAMe T CF(CPAMD8 / FAMe T) area, thus can judge the c DNA sequence is ridge tail FAMe T gene sequences of white shrimp. Use of real-time fluorescent quantitative PCR(q RT- PCR) method is analyzed in different tissues, different amount of expression in ovarian development change, found FAMe T in morphological and white shrimp are expressed in the tissues in the body, including muscle FAMe T relative gene expression in the highest, gills, Less FAMe T relative expression in other tissues, and muscles, the relative expression quantity significant differences in the gills. This shows FAMe T may participate in regulation of morphological and white shrimp of a variety of physiological functions.2. FAMe T genes in morphological and white shrimp expression analysis of three consecutive ovarian developmentIn order to study method, acid methyl transferase(Farnesoic acid- O methyltransferase, FAMe T) in morphological and white shrimp the role of the ovaries, the real-time fluorescence quantitative polymerase chain reaction(PCR) analysis bred ovarian different development period of three consecutive phase(phase I to V) jaws, ovary and hepatopancreas FAMe T gene expression regulation. The results show that the FAMe T genes in the gills, heart, muscle, liver pancreas, ovary, jaws and etc in the organization are the expression of stability, which expressed in gills and jaws of amount is significantly higher than other groups; In ovarian development cycle, FAMe T gene expression in jaws, ovarian, liver pancreas quantity is almost the same change trend, from stage I to IV gradually decline, the first expression in the V; In each development period, ovary FAMe T genes expressed in large jaws is the highest, about 20-30 times the amount of expressed in ovary. The results show that large jaws is the main organization of synthetic FAMe T, morphological and white shrimp FAMe T gene in the process of breeding mainly expressed in early-stage ovarian development, can lead to synthesis of MF, promote the ovaries.3. Vg in penaeus chinensis and morphological and white shrimp expression analysis of three consecutive ovarian developmentVitellogenin gene expression in liver pancreas trend is: in stage I to IV gradually rise, IV peak. V after brooding period to a minimum. Results show that the morphological and white shrimp ovarian development process, the body vitellogenin express total present gradually rising trend, the total recovery after ovulation. Through the left you can see, in the various periods of the development of ovarian mainly within the hepatopancreas vitellogenin gene expression, so the hepatopancreas is the main part of the synthesis vitellogenin.Vitellogenin within the hepatopancreas expression level is: phase I to IV in ovarian development, vitellogenin gene expression gradually rise, surged to the highest stage IV. V period dropped to the lowest, for the next phase I of the ovaries., according to the results of penaeus chinensis four times before the ovaries, total body vitellogenin expression gradually rising trend, the total recovery after ovulation. Among them, the phase I to phase III and V, ovarian contributed more vitellogenin only IV, within the hepatopancreas vitellogenin express volume surge, far more than quantity expressed in the ovary, become the main organization of stage IV vitellogenin synthesis.4. The morphological and white shrimp and penaeus ovaries histological observationIn ovarian development cycle, morphological and white shrimp and prawn phase I and phase II, China on the development of the egg in proportion to the number of ovarian cells changes slowly, and in the development phase III, the penaeus chinensis ovaries a sharp rise in the number of mature eggs, accounting for about 70% of the total number of ovarian cells, reached the highest stage IV is about 90%. Contrast, morphological and white shrimp in stage II to IV ovarian development process, the number of mature eggs growth is relatively stable, phase III, only 50% of mature eggs, IV rapidly increased to 90%. Contrast in the process of the ovaries, morphological and white shrimp penaeus chinensis in ovaries and hepatopancreas amount of vitellogenin expressed that morphological and white shrimp vitellogenin in stage I to IV gradually rise, growth is smaller, but the former three period when IV rose sharply, to achieve the highest. And penaeus vitellogenin in ovarian development to achieve high value when the phase III, and IV expression quantity. By comparing the morphological and white shrimp and penaeus vitellogenin trend, the quantity of gene expression and its corresponding the ovaries are basically identical. On the other hand, proves the vitellogenin gene regulation function of the ovaries.