Studies On Wet Bubble Disease Of Mushroom Caused By The Mycoparasite Mycogone Perniciosa

In order to further understand fungal pathogens of Mycogone perniciosa(Magnus) Delacroix which cause mushroom wet bubble disease. The pathogen culture characteristic, host range and its control has been investigated. The purpose of provide a reasonable theoretical and practical point of view for ecological control of mushroom wet bubble disease.The results show that SD007 mycelium grew the fastest under red light, and the other three grew the fastest with the alternation of day and night, short- wave light(blue light) could inhibit mycelia growth and conidium germination; Hypha growth rate and chlamydospore yield significantly decreased with increasing Na Cl concentration. Na Cl solution the most significantly inhibited HN0015. All conidia almost did not germinate when Na Cl concentration was 24g/L.We studied interactions between Mycogone perniciosa from mushroom(A. bisporus) and some edible fungi. Using hyphal interference, fermentation filtrate, and fruiting body dipping sauce culture. The results showed that Flammulina velutipes, Pleurotus ostreatus, A. bisporus, Pleurotus citrinopileatus and Pleurotus eryngii were the host of M. perniciosa with exception of Lentinula edodes,. The pathogen had the strongest virulence to P. ostreatus, P. citrinopileatus, A.bisporus, Flammulina velutipes, and the weakest virulence to P. eryngii. Fruiting body water extract of P. ostreatus, A. bisporus and P. eryngii promoted mycelium growth of the pathogen, but L. edodes, Ganoderma lingzhi fruiting body water extract inhibited the mycelium growth of M. perniciosa. Pathogen fermented liquid promoted mycelium growth of the edible fungi.Six microbe fungicides were screened in vitro to determine the abilities of these substances to inhibit the growth of M. perniciosa. Trichoderma spp. and Zhongsheng mycin were found to substantially inhibit the growth of the pathogen, while demonstrating lower toxicity towords A.bisporus than any of other fungicide tested.A preliminary in vivo trial using M. perniciosa-inoculated casings revealed that the preventative use of Trichoderma spp. was able to control the development of disease.