Effects Of N-acetylcysteine On Antioxidative Capacity, Energy Status And Intestinal Mucosal Morphological Stracture In Ducks

This study determined weather supplementation with N-acetylcysteine (NAC) could affect antioxidative capacity, ergy status and intestinal mucosal morphological structure on ducks. Ninety one-day-older cherry valley ducks were randomly allocated into control group,500 mg/kg NAC group and 1000 mg/kg NAC group. Each treatment had 3 replicate pens with 10 ducks per pen. The control group was fed abased diet,500 mg/kg NAC group fed based diet+500 mg/kg NAC,1000 mg/kg NAC group fed based diet +1000 mg/kg NAC. On day 22 of the trial, each treatment randomly chose eight ducks slaughtered to obtain plasma, liver and the intestinal mucosa. The results of the trial as follows:1. The effect of N-acetylcysteine on plasma, liver and intestinal mucosa antioxidative capacity in ducks.(1) Compared to the control group, dietary supplementation with 500 mg/kg NAC decreased (P<0.05) the values of malondialdehyde (MDA) in plasma and jejunal mucosa, decreased (P<0.05) the concentrations of hydrogen peroxide (H2O2) both in the liver and jejunal mucosa, increased (P<0.05) the activities of glutathione peroxidase (GSH-Px) both in the liver and jejunal mucosa, increased (P<0.05) the activities of superoxide dismutase (SOD) in the liver and increased (P<0.05) the activities of catalase (CAT) in plasma and the liver; In comparison with control group, dietary supplementation with 1000 mg/kg NAC decreased (P<0.05) the values of malondialdehyde (MDA) in the liver, jejunal mucosa and ileal mucosa, decreased (P<0.05) the concentrations of hydrogen peroxide (H2O2) both in the liver and jejunal mucosa, increased (P<0.05) the activities of glutathione peroxidase (GSH-Px) in the liver, increased (P<0.05) the activities of superoxide dismutase (SOD) in plasma and the liver, as well as increased (P<0.05) the activities of catalase (CAT) in plasma, the liver, jejunal mucosa and ileal mucosa; In comparison with 500 mg/kg NAC group, dietary supplementation with 1000 mg/kg NAC decreased (P<0.05) the values of malondialdehyde (MDA) in the liver and jejunal mucosa, increased (P<0.05) the activities of SOD, GSH-Px in the liver and increased (P<0.05) the activities of catalase (CAT) in jejunal mucosa. (2) Compared to the control group, dietary supplementation with 500 mg/kg NAC decreased (P<0.05) the levels of HIF-1 mRNA in the liver, jejunal mucosa and ileal mucosa, as well as decreased (P<0.05) the levels of XOR mRNA in the liver and jejunal mucosa; In comparison with control group, dietary supplementation with 1000 mg/kg NAC decreased (P<0.05) the levels of HIF-1 and XOR mRNA in the liver, jejunal mucosa and ileal mucosa; In comparison with 500 mg/kg NAC group, dietary supplementation with 1000 mg/kg NAC decreased (P<0.05) the levels of XOR mRNA in the liver.2. The effect of N-acetylcysteine on liver and intestinal mucosa energy status of ducks.(1) Compared to the control group, dietary supplementation with 500 mg/kg NAC increased (P<0.05) the ATP and ADP in the liver and duodenal mucosa, increased (P<0.05) TAN in duodenal mucosa, as well as increased (P<0.05) EC in the liver, decreased (P<0.05) the AMP and the ratio of AMP to ATP in jejunal mucosa; In comparison with control group, diet contaning 1000 mg/kg NAC increased (P<0.05) the ATP in liver and jejunal mucosa, increased (P<0.05) theADP and TAN in duodenal mucosa, as well as increased (P<0.05) EC in the liver and jejunal mucosa, decreased (P<0.05) the AMP in jejunal mucosa and decreased (P<0.05) the ratio of AMP to ATP in the liver and jejunal mucosa; In comparison with 500mg/kg NAC group, dietary supplementation with 1000mg/kg NAC increased (P<0.05) the ATP, ADP, TAN and EC in jejunal mucosa, and increased (P<0.05) ADP in duodenal mucosa; In comparison with 1000 mg/kg NAC group, dietary supplementation with 500 mg/kg NAC increased (P<0.05) the ATP in duodenal mucosa and decreased (P<0.05) the AMP and the ratio of AMP to ATP in duodenal mucosa. (2) Compared to the control group, dietary supplementation with 500 mg/kg NAC decreased (P<0.05) the levels of AMPK-α1 mRNA in the liver; In comparison with 1000 mg/kg NAC group, dietary supplementation with 500 mg/kg NACdecreased (P<0.05) the levels of AMPK-α1 mRNA in the liver.3. The effect of N-acetylcysteine growth performance and intestinal mucosal morphological structure on ducks.(1) Dietary supplementation with 1000 mg/kg of NAC compared with control group decreased (P<0.1) the ratio of feed to gain 3.3%. (2) Compared to the control group, dietary supplementation with 500 mg/kg NAC and 1000 mg/kg of NAC were both increased (P<0.05) villus height and the ratio of villus height to crypt depth in duodenal mucosa, jejunal mucosa and ileum mucosa, decreased (P<0.05) crypt depth in duodenal mucosa, jejunal mucosa and ileum mucosa; In comparison with 500 mg/kg NAC group, dietary supplementation with 1000 mg/kg NAC increased (P<0.05) the ratio of villus height to crypt depth in duodenal mucosa. (3) Compared to the control group, dietary supplementation with 500 mg/kg NAC and 1000 mg/kg NAC were both decreased (P<0.05) the levels of Caspase-3 mRNA in jejunal mucosa and ileum mucosa.In conclusion, dietary supplementation with NAC improving antioxidative capacity, energy status and intestinal mucosal morphological structure via regulating HIF-1, XOR, Caspase-3 and AMPK-al signaling on ducks.