Metabolites Analysis Of Goat Grass Exposed To Drought Stress And Preliminary Physiological Effect Of Propionic Acid On Wheat Drought-resistance

In order to establish the water-saving and drought-resistance chemical control technology which based on the metabolic response of winter wheat, and initially clarify its physiological mechanisms, we took the highly drought-resistant material Aegilops as the sample to identify the different metabolites on drought-effects, and studied the regulation effects of some of the metabolites on the drought-resistance of common wheat and its preliminary physiological mechanism, the main findings are as follows:Take 16 common wheat varieties and their relatives as the materials, applied indoor potted method, and according to the content of biomass and chlorophyll, we screened out the most drought-resistance material as Aegilops, with metabolomics methods and the using GS-MS technique, we compared the differences in metabolites in the leaves of Aegilops under drought condition and under normal moisture conditions, and screened and identified 38 kinds of differences in metabolites, compared with the normal moisture conditions, there is an increase of 16 substances, a decrease of 22 substances.We selected five kinds of organic acids from the different metabolites, glutamic acid, phenylacetic acid, palmitic acid, butyric acid, propionic, with the application of indoor Hydroponics-add method, we verified its regulatory effect on the drought-resistance of ordinary cultivated wheat and its optimal concentrations, it manifested in the increase of the biomass and chlorophyll content. Tests showed that in the five kinds of organic acids, the 0.25 mmol·L-1 propionate obviously enhanced the effects of the drought resistance of wheat. Further studies of the effects of exogenous acid on nitrogen metabolism under simulated drought conditions, the results showed that propionate can significantly promote the nitrate reductase activity of wheat seedlings under drought conditions, glutamine synthetase activity and glutamate dehydrogenase activity has increased, as well as the nitrogen content of soluble protein and seedlings.A preliminary study under field conditions indicates the effect of propionate on wheat yield formation process in the drought-hit winter, the results showed that under drought conditions spraying propionate can significantly increase chlorophyll content and plant biomass and improve filling rate at the grain filling stage; under normal moisture conditions, there is no significant effect on the quantity of wheat spike, grains per spike, grain weight and yield when spraying water and propionate, under drought conditions, there is also no significant effect on the quantity of wheat spike, grains per spike, grain weight and yield when spraying water, while spraying propionate significantly increased the yield of winter wheat spike and yield, yield increased by 16.7%.