The Association Analysis For Pod Dehiscence And Functional Analysis For Related Gene GmSHPa

Fruit development and maturation is an important part of plants growth,which has closely relationship with the yield and quality of agricultural crops, thereby affecting the economic. Timely pod dehiscence is conducive to collect seed, premature cracking or not cracking will affect the harvest of agricultural products. The pod dehiscence is one of the important factors affecting the yield of soybean when harvested. Therefore, understanding the molecular mechanism of pod dehiscence is propitious to the selection and breeding varieties of resistance to shatter, accelerate the good agronomic traits of wild species enter into cultivated breeding lines. In order to explore the genetic basis of soybean pod dehiscence, the research carry out correlation analysis through using soybean cultivars group and linkage analysis using recombinant inbred lines, finally identifying Preliminarily QTL locus of soybean pods. To further analyze related genes GmSHPα functions of pod dehiscence, this study applied the yeast two-hybrid technology, bimolecular fluorescence complementation technology and quantitative analysis techniques to explore function of GmSHPa. The main results are as follows;1. QTL analysis of pod dehiscence were conducted by CIM method using recombinant inbred lines （RIL）（Nannongl138-2×kefengl） as materials, two additive QTL including qpd16-1 and qpd16-2 distributed on 16 chromosomes were identified. Qpd16-1 was located between sct₀01-satt215 mark, qpd16-2 was located between satt215-satt249 tag. We can locate key genes by the development of new markers in the future. The research carried out association analysis on pod dehiscence through using soybean cultivars group by Q+K hybrid model. We scanned the whole genome with 1142 SNP markers （p<0.01）, nine marks were detected in 2012 while other nine marks were detected in 2013. But these markers have not been to tested repeatly in two years, the reason may be experimental error or interaction of genotype and environment.2. To further explore related genes GmSHPa functions of the pods dehiscence, we used yeast two-hybrid technique to screen GmSHPa-interacted proteins. We builded GmSHPa bait vector and soybeans flower cDNA library vector to conduct yeast two-hybrid experiments. This experiment screened two proteins interacted with GmSHPa, they were GmMADS28 and GmMADS29. These two proteins are members of the MADS-BOX family and the results illustrate MADS genes may interact to regulate plant growth and development together.3. In order to verify the genes screened through yeast two-hybrid in vitro, the experiment conducted analysis using bimolecular fluorescence complementation methods. GmSHPa indeed exist interaction with MADS28 and MADS29, in addition, interactions occurred in the nucleus. In our study, we selected a number of downstream genes to analyze expression of these genes in transgenic Arabidopsis, we found that these genes are upregulated. These results illustrate that GmSHPa promote expression of these genes, in order to clarify the relevant expression regulatory mechanisms, we also need analysis by further experiments.