Cloning And Expression And Analysis Of Expression Protein Activity Of Rong Chang Pig And Landrance’s Immune Related Gene FKBP5

Using RACE, qPCR, Western Blot, gene clone, sequencing, protein expression, this study colned the full-length FKBPS cDNA of Rong Chang pig and landrance, detected the FKBP5 expression profile in several tissues of Rong chang pig and landrance, separated and purified FKBP5 protein of Rong chang pig and landrance. And the results are as folloes:1, we colned the full-length FKBP5 cDNA of Rong Chang pig and landrance from their thymus by RACE technique. The full-length of Rong Chang pig consists of 4119 bp, and the CDS of 1374 bp encodes a polypeptide of 458 amino acid residues. In the 5’and 3’of this cDNA also contains a 212bp and 2533 bp non-coding region, respectively. Landrance’s FKBP5 cDNA full-length was 4164bp, and has a CDS of 1374 bp encodes a polypeptide of 458 amino acid residues.In the 5’and 3’of this cDNA also contains a 255 bp and 2535 bp non-coding region, respectively.2, we used RT-PCR and Western Bolt to detect the FKBP5 expression profile in several tissues of Rong chang pig and landrance. "We also detectd the mRNA expression diversity in Rong Chang pig and landrance. The results showed that FKBP5 widely expression in heat, liver, spleen, lungs, lidney, stomach, brain, muscle, gonads and thymus, especially highly abundance in innate immune-related tissues, thymus.In this research, we also found that landrance has higher expression of FKBP5 than Rong Chang pig in thymus.3, We cloened the CDS region of Rong Chang pig and landrance in the pET-28a expression vector, and separated and purified FKBP5 proteins.We also identified FKBP5 protein by using FKBP5 antibody.This results made foundation for our study function of FKBP5 gene. It is important to futher sutudy of the molecular mechanism of resistance breeding pigs.