This experiment was conducted to determine the effects of physically effective neutral detergent fiber on intake, milking performance and blood parameters of dairy cows fed alfalfa hay-based diets with fixed forage proportion under hot weather conditions.25 lactating Holstein dairy cows, averaged milking 18.3+1.2kg/d, were divided into 5 groups(A,B,C,D and E) of 5 cows, according to day in milk, parity and milk yield. Cows were from second and third lactation, and were from 146+25DIM, fed as follows:US alfalfa hay 16.3%, corn silage 14.9%,Chinese wild rye 3.6%,beet pellet 10.0% and concentrate 55.2%(based on DM). The TMR were chemically identical but included different particle length alfalfa hay:1cm,50%1cm+50%4cm,4cm,25%lcm+25% 4cm+50%7cm,7cm. The experiment lasted 50d including 14d for adaptation and 36d for sample collection.The results showed as follows:1.THI lowest averaged 77.72 units,during the whole experimental period (2013.7-2013.9), and all dhe rectal temperature and respiratory frequency were above the normal physiological range of dairy cows,suggested that dairy cows were under heat stress.2. Increasing alfalfa hay particle length had a significant effect on dietary peNDFs.o level(P<0.05),for 12.22%,13.22%,13.91%,14.31%and 14.99% respectively, on peNDF1.18 level (P<0.05), for 23.77%,24.20%,24.28%,24.65%and 25.28% respectively.3. Increasing dietary peNDF1.18 level decreased dry matter intake,the order of DMI effect was 23.77% group>24.20% group>24.28% group>24.65% group>25.28% group, comparied with 23.33% group, the DMI of 25.28% group significantly decreased(.P<0.05).4. Increasing dietary peNDF1.18 level decreased milk yield, the 24.20% group was highest for milk yield, while the 25.28% group was the lowest; dietary peNDF level had a significant effect on milk fat, milk protein (P<0.05),24.28% group were the highest,and 25.28% group were the lowest for milk fat,and 24.28% group were the highest for milk protein, but no significant differences was observed on milk lactose and Feed/Milk (P>0.05).5. There was no obviously differences were observed on Cor or LPS(P>0.05).In conclusion, dietary peNDF level improved the heat-stressed dairy cows milk performance. When the dietary peNDF1.18 level were 24.20%,24.28%,24.28%and 24.20%, the milk yield, milk fat,milk protein and Feed/Milk were maximal. |