The Detection Of Resistance And Plasmid-Mediated ESBLs In Escherichia Coli Strains From Feces Of Pandas And Living Environment

Giant panda are valuable natural resoures.The Protection of giant panda is the common responsibility of all mankind. We found that gastrointestinal disease was the most common cause of mortality in pandas intestinal disease. So far,methods for the control of the disease are mainly based on antibiotics, especially the p-lactam antibiotics. Escherichia coli are now relatively common in healthcare and often exhibit multidrug resistance, severe antimicrobial resistance of Escherichia coli was caused by the overuse and abuse of beta-lactam antibiotics in animal husbandry, which has become a major concern for wild animal health problems in the world. E.coli can be divided into two categories entitled as:pathogens and commensal bacteria. Informations from the resistant surveillance of commensal bacteria can guide the rational use of antimicrobial agents in veterinary medicine.A total of 125 E. coli strains were isolated from Chengdu Giant Panda Breeding Research Base during 2012 to 2013 year. The 96 E. coli strains were isolated from pandas’ feces and the 29 E. coli strains were isolated from pandas living environment.The sensitivity of 8 β-lactam antibiotics and 10 non-β-lactam antimicrobial against 125 strains of E. coli were determined by E-test according to CLSI guidelines. The sensitivity test results showed that the Ampicillin, Cefazolin, Tetracycline, Doxycycline, Trimethoprim/sulfamethoxazole had the higher drug-resistance rate(ranged 30%～75%)isolated from different sources. but the Ceftiofur etl had the lower drug-resistance rate(≤20%).The Escherichia coli from pandas’ feces and living environment to Tetracycline and Cefazolin had a significant difference in drug-resistance rate(P<0.05),but not for ather antibiotic. most isolates exhibited multidrug resistance to (ranged 17～24) antimicrobial agents.125 E. coli strains resistance profiles with a wide distribution.125 strains were indentified to resistant three to thirteen kinds of antibioties,55 antimicmbial resistance profiles were acquired, the predominant drug resistance spectrum is TET/FLO/NOR.ESBLs and AmpC (3-lactamase in E.coli isolates were detected using double disk confirmatory test according to CLSI guidelines and three-dimensional test, respectively. The results showed that 25 isolates isolated from panda feces were ESBLs-producing positive strains,. and the present rate is 26.04%.7 isolates isolated from pandas living environment were ESBLs-producing positive strains,. and the present rate is 24.14%. No isolates were AmpC producing positive strains. The resistant rate of ESBLs-producing strains to CXM,CTX, STR, NOR, FLO, TET, DOX and SXT is signifiantly higher than that of non-ESBLs-Producing strains. The antimicmbial resistance profiles of ESBLs-producing strains is wider than non-ESBLs-Producing strains.The PCR chip was constructed for resistance genes TEM,SHV,CTX-M,OXA,ampC.4 genes encoding ESBLs,1 genes encoding AmpC β-lactamase was detected by PCR and direct PCR sequencing. The PCR results show there were 20 strains TEM,12 strains CTX-M,7 strains OXA,16 strains amp C.Isolates from pandas’ feces harboured TEM、CTX-M、OXA、AmpC, and the present rate is 31.03%. Isolates from pandas’ living environment harboured TEM、CTX-M、AmpC and the present rate is 31.03%.strains from pands showed lower levers of resistance, we should continue to do a good job of feeding and management for protection the rare wild animal, present rate of isolates harboured genes was low,the strains produced ESBLs was popular,in the clinical prevention and treatment use β-lactamase rationally.