| Tobacco mosaic virus (TMV) is a viruse with highly infectious traits and wide range of host plants; it seriously threatened the agricultural production for a long time. Plant activator, a new type of pesticide, is a good and effective comprehensive alternative to protect crops against the various disease especially virus.This thesis mainly researched the induced resistance of N and S containing compounds numbered as 1y3-51-9, 1y3-51-1, 1y5-13-1,1y5-18-1 and 3,4-dichloro isothiazole-5-carboxylic acid. The tobacco infectioned by TMV will generate necrotic lesions on the blades, and then calculate the result by the different necrotic lesions numbers between drug-treated group and blank. In the induction mode, tiadinil (TDL) and isotanil were used as positive controls, while for inactivation, curative activity and protection modes, ningnanmycin was employed as positive control. Green fluorescence protein (GFP) was used as reporter gene to directly and conveniently detect the proliferation and infection of TMV in the host plant. The TMV expression vector inserted with GFP gene, namely p35S:30B:GFP was transformed into Agrobacterium EHA105, which was then innoculated into leaves of Nicotiana benthamiana. Study on three selected modes as inactivation, curative activity and induction. In addition, the insecticide tebufenozide was used as the negative control, and other control drugs were chosen as above.The results of this study indicated that, 1y3-51-9, 1y3-51-1,1y5-13-1,1y5-18-1 and 3,4-dichloroisothiazole-5-carboxylic acid all have systemic acquired resistance, and the induction activity is equivalently to or higher than that of the benzothiadiazole (BTH) and tiadinil (TDL) did except for Iy5-13-1. There are subtle differences between varieties of tobacco. Besides, the GFP tracer also indicated that the efficacy can last about 3 weeks, when efficacy was better; the efficacy lasting time was longer. The results have direction to the novel pesticide development. |
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