Detection Of Reticuloendotheliosis Virus And Avian Leukosisi Virus In Chinese Local Breeds And Investigation Of Its Relationship With Vaccines Used On Them

Avian Reticuloendotheliosis Virus(Rev) and avian leukosis virus(ALV) is a bird of the two kind of retrovirus. They can not only caused by infection of chickens tumorigenesis and immune suppression, but also because the chicken group vertical propagation caused many disputes. In addition, these two viruses are the most frequent exogenous viral contaminants in the attenuated vaccine. In this study, we investigated the REV and ALV infection in ten kinds of local strains of chicken, and made use of a variety of methods for the detection of ALV and REV.1500 ELISA antibody detection kit and indirect immunofluorescence(IFA) were used to detect ALV-A/B, ALV-J and REV antibodies in 1 serum samples from ten local strains. The results showed that the positive rates of REV, ALV-A/B and ALV-J were 100%, 8%, 17.3%, 4%, 16%, 59.3%, respectively.On the whole, by ELISA for serum samples positive by IFA review of test were positive for, but some by ELISA detection OD value of the readings in the critical value in the vicinity of the serum samples by IFA visible typical green fluorescence can also be judged to be Rev antibody positive. IFA sensitivity higher.Rev, and ALV-A/B Rev for 35 days after(AIV-H9. Different breeder flocks of Newcastle disease virus(NDV) inactivated vaccine and H9 subtype avian influenza virus inactivated vaccine, chicken Marek’s disease virus(MDV) attenuated vaccine, fowlpox virus(FPV) attenuated vaccine, NDV avirulent vaccine, chicken infectious bursa of Fabricius virus(IBDV) attenuated vaccine respectively according to the Chinese Veterinary Pharmacopoeia requirements inoculated SPF chickens and 42 d detection of ALV-J antibody, the results displayed in the four kind of attenuated vaccine antibody were negative, but in some batches of Newcastle disease virus(NDV) inactivated vaccine in the detection of Rev antibody display preparation for preparation of chick embryo possible pollution. REV and REV were detected by conventional RT-PCR assay, PCR combined with nucleic acid probe detection, and ALV and ALV were detected by and PCR. The results showed that REV and ALV were detected by dot blot hybridization assay.REV and ALV were detected by conventional RT-PCR assay. Two methods for different batches of four kinds of vaccine testing results were negative, and the detection results are consistent with the SPFvaccination.