Effect Study Of Eutrophic Water On Quality Of Sparganium Stoloniferum

Environment has an important effect on the formation of Chinese medicine quality, and the aquatic environment plays a crucial role in the aquatic medicinal plants. So there is an important significance on studying the change of the aquatic plant growth and development characteristics, physiological-biochemical characteristic and revealing the aquatic herbs quality forming mechanism. We studied the Sparganium stoloniferum quality formation and change under the the condition of eutrophication, using the methods of Plant physiology, plant metabolomics and proteomics environment, analytical chemistry and pharmacology, etc. to explore how the eutrophic water affect the physiological-biochemical characteristic and secondary metabolite of Sparganium stoloniferum, for laying a solid foundation on fully revealing the formation of the Sparganium stoloniferum quality.There is a great influence on the aquatic plant physiology and biochemical composition, so we determined the physiological and biochemical indicators of Sparganium stoloniferum under the different condition of eutrophication after 30 days cultivating, and the results show that there is a significant promoting role in relative growth rate, soluble protein content, chlorophy content, plasma membrane permeability and the activity of CAT and PPO when the nitrogen and phosphorus concentrations increased. Meanwhile, there is an inbibitional effect on soluble sugar content, proline content, MDA content and the activity of POD when the nitrogen and phosphorus concentrations increased, and there is no significant role in the activity of SOD, so we can think that there are different effects on the growth and development of Sparganium stoloniferum under different eutrophication treatment.We determined the related content of secondary metabolites and the activity of PAL, C4H, 4CL of Sparganium stoloniferum under the different condition of eutrophication after 30 days cultivating.The results show that there is a significant promoting role in the content of total phenolics and total saponins in the experimental group when the nitrogen and phosphorus concentrations increased. Meanwhile, in the control group, the contents of total phenolics and total saponins are the highest, so we can think that here is a significant promoting role in the synthesis and accumulation of total phenolics and total saponins at the beginning of the growth of Sparganium stoloniferum under poor nutrition stress. From the results of seven phenolic components content and the activity of key enzymes, we can also know that in the control group,the content of seven phenolic components and the activity of PAL and 4CL are highest.The results show that the secondary metabolic processes of Sparganium stoloniferum were activated under poor nutrition stress from synthesizing and accumulating the secondary metabolites against poor nutrition stress influence.we determined the physiological and biochemical indicators of Rhizoma Sparganii under the different condition of eutrophication, the results show that there is a significant promoting role in the shape, yield, soluble protein content, soluble sugar content, proline content, MDA content and the activity of CAT, POD and PPO. So we can think that there is a significant impact on the yield and the physiological and biochemical indicators of Rhizoma Sparganii, and good adaptability to nitrogen and phosphorus stress.We determined the related content of secondary metabolites and the activity of PAL, C4H, 4CL of Rhizoma Sparganii under the different condition of eutrophication, nd the results show that there is a significant promoting role in the content of total phenolics when the nitrogen and phosphorus concentrations increased. Meanwhile, there is an inbibitional effect on the content of total saponins. Then, the content of mannitol presents promotion before inhibitory state when the nitrogen and phosphorus concentrations increased. Compared with the early cultivated, the content of three compositions increased significantly.Objecting to analysis of protein expression profiles of Rhizoma Sparganii stem tuber in a eutrophia stress culture system, we established the method of 2 dimensional electrophoresis (2 DE) for proteins assay. The experiment results indicated that when cultured under different concentrations of nitrogen and phosphorus stress, the number of protein isolated from protein expression profile of Rhizoma Sparganii stem tuber was significantly different and presented an increase trend. In different groups, the concentration gradient greater, the number of protein which had different expression in spectrogram were more, indicating that the different concentrations of nitrogen and phosphorus stress had greater influence on both expression levels and kinds of protein in tubers of Rhizoma Sparganii. Applied MALDI-TOF/MS mass spectrometry technique, all 18 differentially expressed protein were identified and in which eight proteins were involved in the process of photosynthesis, four proteins were associated with protein synthesis, two were involved in fatty acid synthesis and decomposition process and one protein were involved in metal ion transport. The result suggested that eutrophia stress had a significant impact on photosynthesis of Rhizoma Sparganii.Based the anticoagulation potency and antioxidant potency bioassay method, we developed a method for quantitative determination of biological activity to determinate the value of biological effects for Rhizoma Sparganii from different regions and species. Results showed that the pharrmacodynamic activity of Rhizoma Sparganii from different regions and species were quite different, and Scirpus yagara had superior anticoagulant activity and antioxidant activity than Sparganium stoloniferum. Usage the established antioxidant potency determination method, we assayed anti-oxidation of Rhizoma Sparganii which were cultured under eutrophication stress. Compared with control group (Rhizoma Sparganii cultured in oligotrophic stress system) whose antioxidant effect was the optimum, biological potency the of Rhizoma Sparganii cultured under median concentration eutrophication stress was the highest and there were any significant differences among all groups. On the bases of the experimental results stated above, we found that the antioxidant activity of Rhizoma Sparganii was sharply impacted by eutrophication stress.