| Haemophilus parasuis disease also known as leather Laser disease, is made up of Haemophilus parasuis (HPS) caused a serious contagious disease. Antibiotic resistance of Haemophilus parasuis disease prone, are often sensitive to the onset of drug, later there will be a tolerance, especially long-term and widespread use of ampicillin in pig farms, has produced drug resistance of the bacteria.To explore the effective treatments of Haemophilus parasuis, more than one drug in the laboratory of Haemophilus parasuis antimicrobial susceptibility testing, according to the results of drug susceptibility testing, chosen third generation quinolone antibiotic enrofloxacin has therapeutic applications in clinical trials.Whole test main is divided into Ⅱ a stage for, a is laboratory pig Deputy bloodthirsty Bacillus bacteria HIV through artificial infection experiment pig, in onset infection 36 hours Hou with 10% between enrofloxacin sand star bare type powder agent, and 10%.between enrofloxacin sand star cysts type, and 10% between enrofloxacin sand star injection to different dose on infection disease pig for corresponding treatment, while determination has between enrofloxacin sand star in body within of concentration level, observation between enrofloxacin sand star on infection disease pig treatment effect; Ⅱ is on suspected occurred pig Deputy bloodthirsty Bacillus disease of farms sampling, reference DB13/T 1216-2010 Deputy pig bloodthirsty Bacillus disease control technology procedures, and NY/T 2417-2013 pig Deputy bloodthirsty Bacillus detection method, standard for has pig Deputy bloodthirsty Bacillus identification, used third generation praziquantel Novo ketone class antibacterial drug 10% between enrofloxacin sand star bare powder formulations, and 10% between enrofloxacin sand star cysts type, and 10% between enrofloxacin sand star injection used different of dose, and different of to drug way, for has between enrofloxacin sand star on pig Deputy bloodthirsty Bacillus disease of clinical application research, Simultaneous determination of enrofloxacin concentration in the organism to investigate what type of enrofloxacin with more or less dose achieved better therapeutic effect, in order to provide scientific basis for control and prevention of Haemophilus parasuis disease. Test as follows:(1) laboratory drug sensitive test results:pig Deputy bloodthirsty Bacillus on between enrofloxacin sand star, and ring c sand star, and head spore Sai injunctions sodium, and o Mo Westwood, and kanamycin, and strong mold pigment, and ammonia benzyl Westwood, and gentamicin hypersensitive, on Thai Le bacteria pigment, and sulfa -6-methyl oxygen pyrimidine, and compound rule bacteria sulfonic, and fluoride benzene nikaozhongmin, on forest can Grand, and Thai wonderful bacteria pigment, and Novo fluoride sand star, and sulfa between methyl oxygen pyrimidine low sensitive.(2) laboratory determination between enrofloxacin sand star in body within concentration, judge between enrofloxacin sand star drug in body within peak value on pig Deputy bloodthirsty Bacillus sterilization capacity and effect:taking between enrofloxacin sand star drug Hou, by each group random each 2 head total extraction 24 head pig, collection liver, and kidney, and heart, organization organ, to between enrofloxacin sand star detection reagents box through enzyme marked instrument for 2.5 mg/kg and 5 mg/kg of dose between enrofloxacin sand star in organization in the 2 h and 5h of drug concentration of determination.(3) a pair of healthy pigs with artificially infected with Haemophilus bacteria treatment and control effect of enrofloxacin:select 40--60 day 20kg about healthy nursery for 40 hours, divided into 4 groups, each group was 5. Experiment group for using powder formulations between enrofloxacin sand star, dose for 2.5mg/kg, ear, for 1-10,; death 3 head, mortality for 30%, cure rate for 70%,3, and 5th, pig is stiff pig, stopped drug Hou observation 30 days weight average increased heavy 4.5kg/head; test Ⅱ Group powder formulations between enrofloxacin sand star, dose for 5mg/kg, ear, for 11-20,; no death, cure rate for 100%,7, and 8th, pig is stiff pig, control illness Hou, Stopped drug Hou observation test Ⅱ group 30 days weight increased heavy average 5.6kg/head; test Ⅲ group package was formulations between enrofloxacin sand star micro SAC, dose for 2.5mg/kg, ear, for 21-30,; death 1 head, cure rate for 90%,11, and 12th, is weak stiff pig status, stopped drug Hou observation test Ⅲ group 30 days weight increased heavy average 7.6kg/head; test Ⅳ group package was formulations between enrofloxacin sand star micro SAC, ear, for 31-40, dose for 5mg/kg, no death, cure rate for 100%, Observation of Group IV for 30 days after stopping weight gain average 12.3kg/head, the curative effect is best.(4) test Ⅱ in Jiangxi province JI’an city, xingan city sanhuzhen a farms sampling, through laboratory training separation identification diagnosis for pig Deputy bloodthirsty Bacillus disease, with different type and dose of between enrofloxacin sand star on the disease treatment (due to suspected hidden infection ring virus infection, enhanced disease pig of immune capacity special added has Astragalus polysaccharide):is divided into 4 a group, each group for 25 head pig, experiment group for using powder formulations 10% between enrofloxacin sand star +20% Astragalus polysaccharide, dose for 2.5mg/kg, ear, for 1-25,; Experiment Ⅱ group for using powder formulations 10%between enrofloxacin sand star+20% Astragalus polysaccharide, dose for 5mg/kg, ear, for 26-50,; experiment Ⅲ group for 10%package was formulations between enrofloxacin sand star micro SAC+20% Astragalus polysaccharide, dose for 2.5mg/kg, ear, for 51-75,; experiment Ⅳ group for 10% package was formulations between enrofloxacin sand star micro SAC+20% Astragalus polysaccharide, dose for 5mg/kg, ear, for 76-100,.100 sick pigs,85 of them head back to normal; 5 pigs, Anthony, growing slowly; poor 2 pigs recover, long pull a thin stool, slow; the overall cure rate is 92%, the mortality rate was 8%. Experimental group treated the worst, a mortality rate of 80% and recovered slowly in the late experimental group Ⅳ therapy is the best, no death and illness recovery.(5) for by pig Deputy bloodthirsty Bacillus disease caused pig only appetite waste must, spirit gloomy, temperature 41 degrees above pig, muscle note 10% of between enrofloxacin sand star injection to 5mg/kg, used Ⅱ days treatment, found in early tie head spore Sai injunctions using effect best, alone using effect more better, late using in the due to disease pig device mass organ injury serious who, its treatment effect not obviously. Therefore, in cases of Haemophilus parasuis disease, recommends the use of 10% microcapsule coated dosage forms of enrofloxacin, uniform material feeding with 5mg/kg, using 5-7 days, treatment effect is most obvious. |
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