| Burdock (Arctium lappaL.) belongs to the Compositae (Compositae) Arctium (Arctium) biennial herb.Burdock was traditional Chinese drugs in our country, was also the Chinese ancient medicine food and vegetables.Modern clinical pharmacology research shows that the arctigenin and arctiin in fructus arctii has antitumor, antibacterial, antiviral, fall blood sugar and treatment effect such as kidney disease. Burdock root has functions of promoting blood circulation, prevent high blood pressure, anti-aging, clear intestines and stomach garbage, lower cholesteroland so on.Burdock plant height, root system developed, the production of vulnerable to adverse environmental factors such as drought, waterlogging, freezing injury and the effect of high salt stress, thus affect the amount of Fructus Arctii, thereby causing loss to the production.Taking burdock as material, through the determination of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activity, malondialdehyde (MDA) content, transpiration rate (Tr), stomatal conductance (Gs) and net photosynthetic rate (Pn), research the time under different gradient, drought, floods and salt stress effects on burdock physiological characteristics.After selecting stress conditions, studied the physiological changes in JiangSu FengXian, ShanDong JiNan, JiLin YanBian, SiChuan JianYang, Japan five origin burdock under drought, floods and salt stress, provide theory data for burdock adversity resistance mechanism, also provide theoretical basis for burdock resistant varieties screening.The main results were as follows:1. When the concentration of PEG is 20%, nine days isthe highest activity of SOD,6 days is the highest activity of POD,6 days is minimum of MDA content, PEG concentration was 25%,6 days, CAT activityisthe highest. When the concentration of PEG is 20%,6 days, the Tr and Gs, Pn values compared with ck was significant difference, so choose to PEG stress concentration 20%,6 days as a different origin burdock drought stress time simulation conditions. Drought stress burdock, different regions in Si Chuan burdock of SOD, POD activity is higher than other regions, the CAT live is higher also, synergy between antioxidant enzyme system, so the Si Chuan burdock lowest MDA content, higher antioxidant enzyme activity, reduce the stress to the destruction of the chloroplast,The Si Chuan burdock has the highest net photosynthetic rate, photosynthetic capacity is the best. Therefore, Si Chuan burdock drought-resistant ability is one of the best five regions.2. Deal with waterlogging stress in 3 days, SOD, CAT activity is highest,5 days the highest POD activity, minimum 3 days MDA content, MDA content increased with stress time in 3 days. The highest net photosynthetic rate in the third day, net photosynthetic rate reduced gradually after 3 days, so choose 3 days as a different origin waterlogging stress time. Different waterlogging stress of burdock, Si Chuan burdock highest SOD, CAT activity, POD activity is higher also, MDA content is the lowest, Si Chuan burdock net photosynthetic rate is higher. Therefore, Si Chuan burdock flood-resistant ability is strongest 5 regions.3. Salt stress concentration as the tendency for 80 mmol/L, nine days the highest SOD, CAT activity, POD activity in 12 days, tendency for 80mmol/L is highest,9 days, the concentration of salt stress of 80 mmol/L MDA content is the lowest. Salt concentration as the tendency for 80 L, significant difference was found in 9 days burdock net photosynthetic rate and controlled. So the selection of salt concentration tendency for 80 mmol/L, stress time 9 days for different origin burdock salt stress simulation conditions. Salt stress, Si Chuan burdock SOD, POD, CAT activity and MDA content is the lowest, highest Ji Lin burdock and net photosynthetic rate in Si Chuan. Therefore, Si Chuan burdock maximum salt resistance. |
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