Reproductive Regulation By Wolbachia Titer Of Trichogramma Embryophagum(Hartig) And Horizontal Transmission

Wolbachia is widely distributed in the follows genera（Trichogramma spp.） in the wasp, and the interaction between host diversity, complexity and biphasic, so explore the interactions of Wolbachia and insect hosts, to understand the mechanism of host reproductive Wolbachia regulation to maintain ecological safety and biological control application is of great significance.This test is to feed temperature and tetracycline on embryo transgenerational as follows, and through the expression of Wolbachia inside its body drops degree and quantity of research, has been clear about the temperature and the correlation between tetracycline and Wolbachia drop degree and degree of Wolbachia drops on the biological characteristics of host; At the same time part of daqing region lepidoptera and coleoptera insects and feeding relationships in the laboratory and biological communities is used to detect the Wolbachia, preliminary understanding the Wolbachia infection situation in daqing region and horizontal transmission.The main results are as follows:High temperature（25 ℃ or higher） can change the camp parthenogenesis Wolbachia content follows mode of reproduction and the body, and with the increase of temperature and treatment time significantly. Namely high temperature as follows to camp parthenogenesis reproduction way change degree was negatively correlated with their body Wolbachia content,and the intensity of Wolbachia in reproductive way and with the host are related. And only Wolbachia drop degree reaches a certain threshold, the temperature can control the host reproductive way.Different concentration of tetracycline for food germ, the Wolbachia follows the body with different degree of inhibition, and the higher the concentration of tetracycline, the stronger the inhibition. With the increase of concentration of tetracycline, host drone percentage were positively correlated with the host in vivo Wolbachia drop degree, with the increase of process algebra, the correlation between fluctuation is not big; The host for the Wolbachia drops and tetracycline concentration were positively correlated, the linear relationship related with successive transfer culture has little to do.Under 28 ℃ treatment, F₁ F₅ generation feed embryo follows the body Wolbachia wsp amino acid sequence changes, mainly is the amino acid residues of mutations, and with the increase of processing generation, residues of change become more numerous. Under the tetracycline feeding, feed F1 F5 generation embryo as follows in the Wolbachia wsp amino acid residues at the same, there is little mutation of amino acid residues, missing or insert.In daqing region of 17 families, 2 orders of Wolbachia testing results show that 14.3% ofcoleoptera and 20.0% of lepidoptera insects have the Wolbachia infection.Through the MLST method to detect food embryo as follows- rice moth, predator mites-rice moth and red valley stealm Wolbachia in three parasitic moth, feeding relations, found that Wolbachia horizontal transmission between host did not occur.