Soybean Physiology And Differential Expression Protein To Drought Stress

We knew soybean need much water in its whole life,because forming 1g dry m atter need 600-800 g water. The water don’t only effect plant morphological and physiology but also yield and quality, presenting the positive correlation relationship. A two-year pot experiment(from 2014 to 2015) with Heinong44 and Heinong65 was conducted at Northeast Agricultural University.This experiment adopts the weighing method combined with soil moisture measuring instrument to control moisture content, setting up different degree of drought stress(seedling, flowering period, drum) at different periods(mild drought stress, moderate drought stress, severe drought stress).We studied the morphological characteristics, physiological characteristics, yield and proteomics to learn the response of soybean to drought stress, as well as the rewater compensa tion effect. The main conclusions were as follows:1. Osmotic regulation substances content in the leaves increased under drought stress and osmotic regulation substances content showed rising trend, with the increase of the degree of drought stress. After rewater, all of the physiological indexes had the compensation effect and Amount of compensation effect. The compensation effect: mild drought stress, moderate drought stress > severe drought stress.2. Antioxidant enzymes activity and MDA content in the leaves increased under drought stress and antioxidant enzymes activity and MDA content showed rising trend, with the increase of the degree of drought stress. After rewater, Antioxidant enzymes had the compensation effect and Amount of compensation effect. The compensation effect: mild drought stress, moderate drought stress > severe drought stress.3. We screened 2437 proteins in soybean leaves whose p-value were less than 0.05 and the differences of multiples were larger than 2. Differentially expressed proteins in leaves took prat in the most pathways for Metabolic pathways(471 proteins), second was b iosynthesis of secondary metabolites(279 proteins),oxidative phosphorylation(153 proteins), Ribosome(144 proteins), Glycolysis / Gluconeogenesis(53 proteins), Oxidative phosphorylation(51 proteins)and so on.4. With the increase of drought stress degree yield was lower. When the R5 was suffered with drought stress, yield would be the lowest. After drought stress, soybean will have its compensation effect. The degree of compensation effect was associated with the degree of drought stress, and the greater the degree of drought stress, the less compensation effect.