Gene Expression Regulation Of Ankrd23 And Long Non Codingrna—ak004293 During Myoblast Differentiation

Muscle fiber formation is a complex process and subject to fine regulation of a variety of protein-coding genes and non-coding RNA. Based on our previous results, we identified a long non coding RNA(lncRNA)- AK004293 which was highly expressed in muscle and overlapped with the 3’UTR of ANKRD23 gene. In this study, we detected the effect of AK004293 on the expression of ANKRD23 and the effect of these two genes on the expression of myoblast differentiation marker genes in C2C12 myoblasts. The results are as follows:1. Quantitative real-time PCR and Western blotting were used to detect the expression change of myoblast differentiation marker genes and ANKRD23 after knockdown and overexpression of AK004293. The results showed that AK004293 has no significant effect on the expression of myoblast differentiation marker genes and ANKRD23 gene.2. Quantitative real-time PCR and Western blotting were used to detect the expression change of myoblast differentiation marker genes after knockdown and overexpression of ANKRD23. The results showed that the expression of myoblast differentiation marker genes was increased by ANKRD23 interference and the expression of myoblast differentiation marker genes was reduced by ANKRD23 overexpression, indicating that ANKRD23 played a negative regulation role in the myoblast differentiation.The results established the foundation for further studies about the function and mechanism of ANKRD23 in muscle development.