Ffect Of Astragalus Polysaccharides On Mouse Oocytes In Vitro Maturation

In the technology of animal embryo engineering and animal nuclear transfer, oocytes in vitro culture is one of an important thing, which is great significant for improving the efficiency of embryo culture in vitro and improving the application of animal embryo production and promoting the development of assisted reproductive technology. In addition, with the rapid economic development, causing a series of problems, such as environmental pollution, life and work pressure increasing, resulting in more and more people to face the reproductive barriers. The accumulation of reactive oxygen species and the increase of the cytoplasm color of oocytes increased, which greatly reduced the success rate of fertilization. Therefore, it is very important to improve the quality of oocyte. APS has a long history in China, anti-inflammatory, antioxidant, scavenging free radicals have a significant role. But it is not clear how the oocytes cultured in vitro. Also in veterinary clinical medicine, Astragalus injection is often used as an immune enhancer combination therapy with antibiotics, Astragalus drugs in the course of the reproductive performance of dams whether the impact, but also a question need to focus.The aim of this study was to explore the effect and mechanism of Astragalus Polysaccharides on the in vitro culture of oocytes. The acquisition of immature mouse oocytes add Astragalus Polysaccharide(concentration of 0 mg/ml, 0.1 mg/ml, 1 mg/ml, 10 mg/ml) of in vitro maturation(In vitro maturation, IVM) statistical culture, oocyte germinal vesicle breakdown(germinal vesicle breakdown, GVBD) and the incidence of the first polar body(first polarbody,PB1) discharge rate, and mitochondrial membrane potential was detected by oocyte JC-1 staining method; in order to investigate the effect of Astragalus Polysaccharide on mouse oocytes in vitro oxidative damage during the ripening process, this study further in oocytes cultured in liquid with methylglyoxal(Methylglyoxal, MG, the experimental results show that the use of chemical reagents methylglyoxal can build oocyte oxidative damage model, the active oxygen content in cells increased) co cultured with astragalus polysaccharide, statistics the incidence rate of GVBD and PB1 emission rate, To detect the production of reactive oxygen species(oxygen species Reactive, ROS), and to study the effect of Astragalus Polysaccharides on the in vitro culture and in vitro maturation of mouse oocytes. Test results show:(1) During the in vitro maturation of mouse oocytes, the addition of Astragalus polysaccharides showed no significant effect on the in vitro maturation rate of oocytes, including the incidence of GVBD and the rate of PB1 efflux.(2) There was no significant difference in the mitochondrial membrane potential detection between different concentration groups.(3) Add Astragalus Polysaccharide and methylglyoxal cause oxidative damage of oocytes co cultured results show, had significantly lower rates of excretion 10 mg/ml APS+MG experimental group PB1 and corresponding oocyte ROS generation increased significantly, and incidence of GVBD is not significantly affected.The results showed that APS had no significant effect on the in vitro maturation of oocytes. Add MG co-cultured with APS foun,d after 10 mg / ml APS co-culture with MG led to the PBE1 significantly reduced, the corresponding cells was significantly higher ROS production. The results also show that Astragalus drugs in clinical use for the process of reproductive performance of is safe.