| Effective hemostasis is an important problem needed to be solved in surgery, especially in neurosurgery. Common hemostasis can not be used in brain due to fragile tissue, tissue capillary diffuse blood infiltration is more difficult to deal. Therefore, the research for intracranial hemostatic materials and new technology have become important in surgery and veterinary medicine. In addition, the safety of hemostatic materials used in CNS was concerned by researchers, because this is related to the further development and clinical application of hemostatic materials and technology. This experiment choose a new hemostatic material-ShuRiuji(SRJ) as research object, and administrated SRJ in CNS by cerebrospinal fluid replacement, then evaluated the influence of SRJ on main physiological function and the central nervous system, in order to provide basis research for neurosurgery in animal.The research included:(1) explored the influence of SRJ on main physiological function: 24 New Zealand white rabbits were randomly assigned in three groups, saline control group(group N), SRJ group(Group S) and kaolin suspension group(Group G). After establishment of models, observed clinical symptom, monitored heart rate(HR), respiratory rate(RR), pulse oxygen saturation(SpO2) and temperature(T), and measured blood cytology indexes and some biochemical indexes.(2) explored the influence of SRJ on neuroethology: tested by Morris water maze and functional observational battery(FOB).(3) explored the influence of SRJ on oxidative stress level: 45 Wistar rats were were randomly assigned in group N, group S and group G, all rats were put to death at different time points after establishment of model, and tested MDA、GSH-Px、SOD and CAT.(4) explored the influence of SRJ on tissue pathological morphology: 45 Wistar rats were were randomly assigned in group N, group S and group G, all rats were put to death at different time points after establishment of model, the brain were fixed by 10% formalin, then made pathological sections and observed in microscope.The results showed:(1) physiological function monitoring results showed: HR and RR were decreased in all groups, and then gradually recoveried in normal; SpO2 was remained above 95% in all groups; T was stable in group N and S except group G; changes in indexes of complete blood count(CBC) were not significant in three groups; the indexes of liver and kidney function were transiently increased in group N and S, and then gradually recorveied in normal, ALT and ALP were changed significantly in group G.(2) neurobehavioral test results showed: the results of Morris water maze were similar between group N and group S, average escape latency time and times of space exploration were significant higher in group G than that in group N and S(p<0.05); the results of FOB were similar between group N and group S, MMS was transiently increased in behavior function domain in group N and S, and then recorveried in normal, MMS was remained increased in group G.(3) mearments of oxidative stress level showed: in group N and S, MDA was increased at 6h and 12 h, and then gradually decreased in normal, GSH-Px、SOD and CAT were decresed at 6h,and then gradually increased in nomal; MDA was remained increased than baseline(p<0.05) in group G, GSH-Px、SOD and CAT were decreased continuously.(4) brain tissue pathological observation showed: there was no changes in morphology of brain tissue in group N and S, brain cell was edema signicantly in group G.Comprehensive test results, SRJ has few influence on main physiological function, it will not cause changes of neurological function, oxidative stress and brain tissue morphology, it can be used in CNS with high security. |
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