Study On Attractant Effect Of Rice Volatiles On Chilo Suppressalis And Rice Parasitoid Wasps

Plant volatiles are volatile secondary metabolites from plant, including hydrocarbons, alcohols, aldehydes, ketones, esters, organic acid, nitrogen compounds, sulphur compounds and terpene compounds. In the long-term coevolution, rice (Oryza sativa) has developed to utilize its volatiles to affect the behaviors of herbivores, such as the orientation of the host, feeding, oviposition and repellant behaviors. In addition, rice volatiles are attractive to herbivores’enemies, and can regulate the relationships between herbivores and their enemies especially the volatiles induced by herbivores. Thus, it has become the hot topic to utilize volatiles to manage pest. In this paper, single volatiles’effects to indoor populations of one herbivore, Chilo suppressalis and two parasitic wasps were measured. The research contents are below:1. The improvement of the rearing method in laboratory of C. suppressalis and the study of the effects of several compounds to C. suppressalis adults:the larvas of C. suppressalis reared by water bamboo and artificial fodder showed the highest activity; the behavior experiments indicated that rice volatiles and two terpenoids, linalool (0.1 μg/Kg,100 μg/Kg) and β-caryophyllene (0.1 μg/Kg,1 μg/Kg,10 μg/Kg,100 μg/Kg) have attractive effects to C. suppressalis adults.2. The exploration of the continuous rearing method in laboratory of Apanteles chilonis Munakata and the study of the effects of several compounds to A. chilonis adults:the continuous rearing method in laboratory of A. chilonis was based on the parasition of A. chilonis on the larvae of C. suppressalis, which can rear large population of A. chilonis in high activity; the behavior experiments indicated that the effects choosing rate of trans-3-Propyalacrolein (0.1 μg/Kg,1 μg/Kg), linalool (0.1 μg/Kg,1 μg/Kg), β-caryophyllene (0.1 μg/Kg,10 μg/Kg,50 μg/Kg), ocimene (0.1 μg/Kg,10 μg/Kg) and 2-nonyl ketone (10 μg/Kg) were significant different to the control.3. The improvement of the rearing method in laboratory of Anagrus nilaparvatae and the study of the effects of several compounds to A. nilaparvatae:A. nilaparvatae was reared in a simulated field environment including rice seedling, A. nilaparvatae and Nilaparvata legen to mix all instars of A. nilaparvatae to satisfy the requirement of the experiment; the behavior experiments indicated that A. nilaparvatae had a significant tendency to maize powder and seven compounds can affect its behaviors significantly including trans-3-Propyalacrolein (50 μg/Kg), anti-3-hexene aldehyde (1 μg/Kg,10 μg/Kg,50 μg/Kg), linalool (0.1 μg/Kg,1 μg/Kg, 10 μg/Kg), p-caryophyllene (0.1 μg/Kg,50 μg/Kg), ocimene (1 μg/Kg), α-pinene (1 μg/Kg), methyl salicylate (0.1 μg/Kg,1 μg/Kg,10 μg/Kg,50 μg/Kg).These results demonstrated that large population was the key point of indoor experiments, and there were active components in rice volatiles that can affect the behavior of rice pests and their enemies. The study of these active components in further may provide a new method of rice pest management.