The Exploration Of Proteins Related To The Interaction Between Plasmodiophora Brassicae And Chinese Cabbage (Brassica Rapa L. Ssp. Pekinensis)

The interactions of plants and pathogens can cause defense response, mediated by salicylic acid (SA), jasmonic acid (JA) and ethylene (ET). In this research, the differentially expressed proteins in the root of Brassica rapa L. infected by clubroot were analyzed using the traditional classic two-dimensional gel electrophoresis (2-DE). We are trying to find the proteins participated in the signal pathways of defense response, and to provide new clues for clubroot defense in Brassica rapa L. The main results are as follows:1. The results of microscopic preforming technology showed that at 5 days after inoculation (DAI) with plasmodiophora brassicae, the root hair of most of the experimental materials were infected. So, the 5th DAI was used as the early infected stage.2. Through the optimization experiment on the dosage of root system and protein sample amount, we found that using the Trizol precipitation method to extract the proteins of Brassica rapa L. at least 1.5g root for 2-DE experiment,1000 μg protein sample is suitable for 2-DE.3. Through the 2-DE experiment of the both infected and uninfected root of Brassica rapa L., and the analysis by PDQuest 8.0.1 software, we got the repeated electrophoretogram which have more than 400 proteins.48 proteins loci were found because their variance abundance is more than 2 times. Among those,19 proteins were selected and identified by mass spectrom. It showed 18 differential proteins with over 95% protein score C.I.%. were identified successfully.4.18 differential proteins were analysed by Gene Ontology,39% of them responsed to stimulation and 8% were related to plant defence reaction in the biological process. Among these,3 proteins related to the JA/ET mediated defense reaction. Besides, the GO analysis found a PR-1 related protein in the extracellular cell component area and KEGG analysis indicated a different protein participated in the plant-pathogen interaction signaling pathways.5. Bioinformatics analysis indicated that Brassica rapa L. was infected by plasmodiophora brassicae, It is possible that the differentially expressed proteins 3414、 2358 participated in JA/ET mediated induced systemic resistance. Besides,3038、3129 participated in SA mediated systemic acquired resistance. The differentially expressed protein 4043 participated in both systemic acquired resistance and induced systemic resistance. It also confirmed the existence of a link between these two pathways, rather than an isolated response.