Identification Of Parasitic Nematodes In Greenland Turfgrass Of Guangzhou City

Urban green lawn has become an important and indispensable part of modern urban living environment. With the improvement of people’s living standard, people’s demand for the quality of turf grass is getting higher and higher. Nematodes are one of the important factors affecting the quality of turf grass. To understand species of parasitic nematodes from the turfgrasses is of importance for effective control of turf grass nematode diseases, and for healthy growth of turf grasses.In this study, soil samples from the rhizosphere of different kinds of turf grasses in 14 parks in Guangzhou area were collected. Plant parasitic nematodes were identified based on combination of morphological and molecular characterization. At the same time, phylogenetic relationships of the nematodes based on rDNA subunit (LSU) sequences of the D2-D3 fragment were analyzed. The following main results were obtained:1、Combining morphological and molecular biological characteristics of nematodes, a total of six species of plant parasitic nematodes were identified, including Tylenchorhynchus leviterminalis、Helicotylenchus dihystera、 Mesocriconema xenoplax、Hemicriconemoides chitwoodi、Xiphinema chambersi、 Xiphinema americanum sensus lato、two species of plant parasitic nematodes were also identified according to the morphological characteristics, they are Pratylenchus sp.and Meloidogyne sp.. Among them, T. leviterminalis, H. dihystera、M. xenoplax are the dominant species. T. leviterminalis、M. xenoplax、H. chitwoodi、X. chambersi and X.americanum sensus lato were new records from turf grasses in Guangzhou and China.2、Molecular phylogenetic analysis showed that:Helicotylenchus dihystera GZ in this study was grouped in a clade with other seven H. dihystera populations with 99% support. Mesocriconema xenoplax was clustered in a branch with 18 other populations of M. xenoplax with 85% support, and it was sister to a M. xenoplax population from Fujian longan rhizosphere. Hemicriconemoides chitwoodi was grouped in a clade with three other populations of H. chitwoodi with 99% of support. Xiphinema chambersi was grouped in a clade with two other populations of X. chambersi with 100% support. Xiphinema americanum sensus lato was clustered in a branch with five other populations of X. americanum sensus lato with 89% support.