Phylogeny Of Eurasian Clade Of Vitis (Vitaceae) Based On RPP13

The grapes have abundant ornamental value and economic value, and the Wild grapes have a wealth of agronomic traits, which can provide stocks and resistance gene resources for the breeding of high quality varieties. China is one of the most rich countries in wild grape germplasm resources, so finding out the species of wild grapes and the phylogenetic relationship between them, and collecting and preserving them, is of great significance. This research studied the phylogenetic relationship of 29 Eurasian lineage material of Vitis by nuclear gene DNA sequence analysis, which contain 25species(varieties) in our country, one kind of Europe and Asia, two American species and1 outside groups of Vitis. The results are as follows:(1) This study divided RPP13 gene into fragment a1, a2, a3, then went on sequence aligment for the 3 fragments of 29 Eurasian lineage material of Vitis, and constructed phylogenetic trees. The results show that, the phylogenetic relationship of of Vitis is consistent with the geographical distribution, and the vitis divided into Subgen.Muscadinia Planch. and Subgen. Euvitis Planch.(2) The conjoint analysis of phylogenetic trees of 3 fragments conclude: The sepecies that the genetic relationship is close are Vitis. pseudoreticulata and Vitis. flexuosa,Vitis.romanetii and Vitis.shenxiensis, Vitis.piasezkii and Vitis.hancockii, Vitis.balanseana andVitis.betulifolia, Vitis.chunganensis and Vitis.lanceolatifoliosa, Vitis.erythrophylla andVitis. ruyuanensis.(3) Because of the gene conflicts and choices, the phylogenetic trees of 3 fragments are collapsed, which just slove part relationships. So we shoud choose more suitable genes and go on the conjoint analysis combining multiple genes, which can further to slove the the phylogenetic relationship.(4) RPP13 gene has resistance to downy mildew, which has a definite relation with the phylogenetic relationship of the wild grapes. So this can play a supporting role.