Effect Of Different Diets On Tan Sheep Tail Fat Deposition And Gene Expressions Related To Fat Metabolism

Tan sheep has long fat tail, plenty of fat deposited in the tail. Studied the effect of different energy and protein diets on Tan sheep tail-fat deposition, and the different expression of gene. There were an important role in explore the Tan sheep fat deposition mechanism and improve Tan sheep production performance. This study based on 4~8 months Tan sheep, divided the feeding trails into three stages, aimed to find the changes of fat deposition on different diets. Analysed the genes expression in different energy and protein levels, different stages and the different tissues on Tan sheep, aimed to explore tail-fat deposition mechansim, improve the performance of production based on the optimal feeding formula on Tan sheep, save the cost of feed.This study selected 112 healthy 4~8 months Tan sheep, designed four different energy and protein level diets and on the same DE/CP, namely 0.84 standard level, 0.96 standard level, 1.08 standard level and 1.20 standard level, standard reference China mutton sheep breeding standards(NY/T816-2004). Test was divide into three stages: the first stage 22 ~ 28 kg, the second phase 29 ~ 35 kg, the third stage 36 ~ 41 kg, each stage of four different diet groups kept the same DE/CP(MJ/kg / %), respectively: 0.8, 0.9, 0.88. Measured tail weight, GR-value and tail-weight/carcass-weight, and measured the tail width, tail length, tail basal perimeter, tail middle perimeter, tail thickness. Detected the genes expression of Leptin, ADIG, APn, ADRB3, BTG2, PLIN1 and GLUT4 with real-time fluorescent quantitative PCR technique and made related analysis. The results showed that:1. With the increase of diet energy and protein levels, Tan sheep tail-fat deposition had significant difference(P<0.05), the fat deposition of 1.08 standard level respectively higher 13.11%, 6.43% than 0.84 and 0.96 standard levels, 1.20 standard level respectively higher 28.14%, 20.57%, 13.29% than 0.84, 0.96 and 1.08 standard levels.2. Nutrition levels and Tan sheep tail fat deposition were significantly positive correlation(P<0.05). Nutrition levels and tail-weight/carcass-weight were significantly negative correlation(P<0.05). High energy and protein level diet was beneficial to Tan sheep tail fat deposition. And appropriately raise the level of Tan sheep diet energy and protein were beneficial to reduce the tail-weight/carcass weight, helped to improve Tan sheep production performance.3. With the incraesing of diet energy and protein levels, and the incresing of Tan sheep age, the expression of genes Leptin, ADIG, APn, ADRB3 had a significant difference(P<0.05), and had a downward trend after rising first. The genes inhibited fat deposition, and more fat deposited in the later growth stage.4. With the incraesing of diet energy and protein levels, and the incresing of Tan sheep age, the genes expression of BTG2, PLIN1 and GLUT4 had a significant difference(P<0.05), and had to fall after rise trend. The fatty deposits of the late growth stage promoting effect was greater than the early growth stage, and more fat deposited in the later growth stage.5. In different fatty tissues, the expression of gens Leptin, ADIG, APn, ADRB3 from high to low were as follow: subcutaneous fat > perirenal fat > tail fat, the expression of BTG2, PLIN1 and GLUT4 were as follow: tail fat > subcutaneous fat > perirenal fat. Fat depositon tended to on tail and the least on subcutaneous fat.(6) There were positive correlation between the genes Leptin, ADIG, APn, ADRB3 as well as on genes BTG2, PLIN1 and GLUT4, and genes expression of Leptin, ADIG, APn, ADRB3 were negative correlation to genes expression of BTG2, PLIN1 and GLUT4. The correlation between the expressions of each gene showed that among the expression of genes may be influence each other, they can effect the same tissue fat deposition through different mechanism.6. There were positive correlation between the genes Leptin, ADIG, APn, ADRB3 as well as on genes BTG2, PLIN1 and GLUT4, and genes expression of Leptin, ADIG, APn, ADRB3 were negative correlation to genes expression of BTG2, PLIN1 and GLUT4. They were regulate fat deposition and degradation together.