Establishment Of Detection Method Of Deoxynivalenol

Animal product safety is a hot spot in the current breeding industry. It is very important to control the safety of the products. Mycotoxins pose a great threat to the health of human and animal. The toxin can cause vomiting due to a small amount of pig, so also known as deoxynivalenol. It was considered hazardous to grain or feed common in one of the most dangerous substance, and vomiting toxin contamination of grain and feed in the global scope of the most common, the situation is more severe. The toxin can cause acute, chronic, cellular and reproductive toxicity, and it was a great threat to the health of animals and people. Therefore, It is urgent to develop a high sensitivity and rapid analysis method to detect the residue of the vomiting toxin in the feed, And currently on the market commonly used detection methods, such as colloidal gold rapid detection test strip, ELISA test kit quality is uneven, urgent need to improve and seek new detection method applied to clinical. In this paper, two methods were established for the determination of residues of the toxin of Fusarium, And completed the preparation of the kit to ensure the healthy and orderly development of animal husbandry on the basis.The objective of this work was to establish two detection methods for the detection of DON by indirect competition, respectively, enzyme linked immunosorbent assay and chemiluminescence enzyme immunoassay. The reaction conditions were optimized: First, the optimum proportion of antigen antibody was determined by checkerboard method. Second, the optimum conditions were measured by optimalization of the buffer system and reaction pattern; Third, The detection limits were determined by the appropriate standard curve which was established through all the conditions above; Fourth, the appropriate recovery method were selected through different test samples; Fifth, the various performance indicators of the kit were tested, including repeatability, stability and the existence of cross reaction. Finally, a complete detection method for the detection of DON was established. The detection limits of the kit were determined by the appropriate standard curve which was established through all the conditions above.After optimization, The linear range of the ELISA kit was 10-810 ppb, The limit of detection values(IC15) was 10 ppb, and the sensitivity of reagent kit detection(IC50) is 60.9 ppb; and the recovery rate was 88.7%-116.3%, The whole range of the stability test results is less than 30%.The linear rang of the icCLEIA kit for DON was from 0.5 to40.5 ppb, IC15 was 0.5 ng/mL, IC50 was 1.47 ng/mL, and the recovery rate was 78%-118%, The whole range of the stability test results is less than 30%. Experiments show that the two kinds of reagent kits can be used for the detection of samples. Chemiluminescence enzyme immunoassay can significantly improve the detection limit and sensitivity of the DON kit.