Study On Anti-TMV Activity Of Actinidia Chinensis Planch Root Bark

Plant virus diseases, known as “plant cancer”, which have caused great damage to agricultural industry. Biogenic anti-plant-virus were characterized by environmentally safe due to low residual, safe to non-target organism and degradable. Preliminary study found Actinidia chinensis Planch root bark extract showed activities against Tobacco mosaic virus(TMV). To illustrate anti-TMV active ingredient of Actinidia chinensis Planch root bark extract, TMV were used as the test virus, bioassay-guided fractionation methods were adopted to the isolation and identification of the active compounds. The bioassay of the crude extract and the active ingredients were tested in laboratory. In addition, field plot trials were carried out on polar-guide fractions. The main result are shown as follows:1. 95% Ethanol extract of Actinidia chinensis Planch root bark, suspended with water, was extracted with petroleum ether, ethyl acetate, n-butanol, successively. Half leaf lesion method was used to test the anti-virus activities of the ethanol extract and fractions. After 10-mins and 2-hrs inactivation, the aqueous phase and the ethyl acetate phase showed moderate inactivation of 75.44%, 67.46% and 73.53%, 62.50% under the concentration of 10 mg/mL. The aqueous phase and the ethyl acetate phase showed better inhibiting infectivities, also. In 12-hrs incubation, the inhibition ratio was 54.11%, 42.09% under the concentration of 10 mg/mL2. 7 Compounds were isolated from Actinidia chinensis Planch ethyl acetate extract by diverse separation technology, such as column chromatography, recrystallization, et al. The compounds were identified as catechins-7-phytol(1), 5-methoxy-coumarin-7-β-D-glycosidase(2), catechins(3), fupenzic acid(4), spathodic acid-28-O-β-D-glucopyranoside(5), 3-oxo-9(11), 12-diene-30-oic acid(6), 3-β-(2-carboxy benzoyloxy) oleanolic acid(7). Among them,(2) possessed higher inactivating activity, 52.45%, while(7) showed obvious inhibiting infectivity, 51.10% against TMV under the concentration of 1 mg/mL. Compounds(6) and(7) displayed strong inhibiting multiplication ratios of 47.10%、41.43%.3. Field plot experiments of the Actinidia chinensis Planch ethanol extract, ethyl acetate extract, and aqueous phase were carried out. The results indicated that the control effects against TMV were 35.64%, 34.87%, 45.26%, with 100 times dilution of total extract and ethyl acetate extract and 200 times of the aqueous phase for the first time, significantly higher than that of letinan, 30.79%.4. The results suggested that the Actinidia chinensis Planch root bark exhibit good activity against TMV. Compound(2) showed obvious inactivating activities, while(7) showed good inhibiting infectivity activities. The Actinidia chinensis Planch root bark deserves further research, and has great developmental potential of new type of biogenic antiviral agent.