Tissue Culture Of Photinia Fraseri As Well As The Study Of The Three Kinds Of Medicinal Ingredients Of The Photinia Fraseri

Photinia fraseri is commonly used as landscaping plants, and its leaves can be used as traditional chinese medicine. This study is divided into two parts, the first part is about the optimization of photinia tissue culture techniques, and second part is to study the variation of content of flavonoids, ursolic acid and saponins in tissue culture seedlings and other tissues on the basis of tissue culture. We used axillary plant as the explant to established tissue culture regeneration system, and the emphasis is to investigate influence of the formulation ratio of plant hormone in tissue culture on plant growth. Soxhlet extraction is used to optimize extraction conditions of total flavonoids, ursolic acid and total saponins, and used HPLC to determine the content of them, The main results of this paper are as follows:1、Tissue culture results:The best combination of disinfection is using 0.1%Hgcl2to disinfect for 8min. Hormones formula of the medium of primary-cultured Photinia fraseri is MS+2.0mg/L6-BA+0.lmg/LNAA+ sucrose(30g/L) + agar(9g/L), and the PH is 6.0.The best Subculture medium is MS+0.1mg/L NAA+1.0mg/L 6-BA+0.2mg/L KT+0.1mg/L IBA+ sucrose 30 g,+agar 9g,and the PH is 6.0. The most appropriate rooting culture medium is 1/2MS+NAA0.2mg/L+ sucrose30g+agar9g, the PH is6.0.The most appropriate medium for hardening and transplanting is peat soil, vermiculite,perlite, and the ratio is 5:2:1. The transplant survival rate can reach 96%.2 Extraction optimization of flavonoids extraction solvent is 75% ethanol,extraction time is 2h, extraction temperature is 90℃,and feed-liquid ratio is 1:15.extraction optimization of ursolic acid extraction solvent is 75% ethanol, extraction time is 3h, extraction temperature is 90℃,and feed-liquid ratio is 1:30.The experiment of extraction optimization of saponins extraction solvent is 60% ethanol, extraction time is 2.5h, extraction temperature is 60℃,and feed-liquid ratio is 1:20.3、The content of flavonoids, ursolic acid and saponins is measured by using HPLC. Investigated by methodology, theliquid chromatographic hplc method used in this paper applies to the analysis of the three components in Photinia fraseri.By determination of content, in Photinia fraseri, the content of ursolic acid is the highest,follow by saponins, and flavonoids is the least.