| 20 wild soybean,20 landraces and 20 bred cultivars with national geographic representative were used as experimental materials in our study,405 genome-wide distributed functional gene fragments were sequenced and analyzed, the main results are as follows:（1） We obtained about 182Kb DNA sequence and found 1344 SNP in total. The SNP frequency 0 in wild soybean, landraces and bred cultivars is 1.911×10-3,1.155×10-3 and 1.024×10-3 and π is 1.919×103,1.334×10-3 and 1.221×10-3 respectively.39.5%（θ） and 30.5%（π） of genetic diversity had lost during domestication,11.4%（θ） and 8.5%（π） of that had lost during improvement. （2） The reducing rate of genetic diversity in noncoding region is greater than coding region during domestication and improvement, and when further analysis, the rate of that in nonsynonymous and UTR is slowest, then followed by synonymous and intron is fastest. （3） We identified 27 genes subjected to artificial selection during domestication and improvement by Fst outlier analysis among SNP between populations. （4） Phylogenetic analysis among the three soybean populations revealed that cultivated soybean may originated from Huang-Huai regions and middle-south areas. （5） Linkage disequilibrium （LD） is gradually increased in the process of domestication and breeding. The above results have great significance for further understanding the molecular process and mechanism of soybean origin and evolution, and for isolating or identifying key genes control soybean important agronomic or economic traits. |
PDF Full Text Request