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Volatiles Locating Of Peristenus Spretus (Hymenoptera:Braconidae) To Apolygus Lucorum (Hemiptera:Miridae)

Posted on:2017-02-16Degree:MasterType:Thesis
Country:ChinaCandidate:W J DaiFull Text:PDF
GTID:2283330488492108Subject:Agricultural Entomology and Pest Control
Abstract/Summary:PDF Full Text Request
In this study, behavioral responses of Peristenus spretus Chen et van Achterberg to Apolygus lucorum (Meyer-Dur) nymphs and its host plants with different damage levels and development stages were measured by using Y-tube olfactometer. Active compounds which can elicit P. spretus electrophysiological responses were screened out by using gas chromatography-electroantennography (GC-EAD), gas chromatography-mass spectrometer (GC-MS) and EAG analysis. The main results were summarized as follows:The results showed that the 3rd instar nymphs of A. lucorum, and healthy cotton plants had no significant attraction to female P. spretus. Female P. spretus wasps were significantly attracted to damaged cotton plants (both with nymphs removed and with nymphs left), but no difference was found between these two treatments. However, male P. spretus showed no markedly preference to any treatment above mentioned. In addition, comparing with vegetative cotton plants (healthy and damaged with nymphs removed), female A. lucorum adults were significant attracted to flowering castor bean plants with the same treatments, whereas female wasps only preferred damaged castor bean plants but nor for healthy ones. Furthermore, female P. spretus preferred damaged plants (with nymphs removed) of cotton and castor bean at flowering stage than the corresponding vegetative plants. The results showed Female P. spretus mainly utilizes the volatiles from host plants damaged by A. lucorum, and the behavioral responses of female P. spretus to flowering host plants were consistent with that of A.lucorum.In addition, we screened out two active compounds from the collected plant volatiles, including m-Cymene and 4’-Ethylacetophenone. When m-Cymene concentration at 10 mg/ml and 4’-Ethylacetophenone concentration at 100 mg/ml, electrophysiological responses of female P. spretus were the highest. The results showed volatile compositions m-Cymene and 4’-Ethylacetophenone play an important role in host selection of P. spretus.In this paper, We assessed the behavioral responses of the nymphal parasitoid P. spretus to A.lucorum and its host plants, and then identified active compounds which P. Spretus has electrophysiological responses to. It could provide the theoretical basis for effectively controlling A. lucorum using P. spretus.
Keywords/Search Tags:Peristenus spretus, Apolygus lucorum, host plant, plant volatile, behavioral response
PDF Full Text Request
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