The Industrialization Of Silkworm Efficient Additives Applied Research

Silkworm（Bombyx mori）（B. mori） is an important economic insect, and also a mode of lepidoptera insects. Silkworm has a history of more than 5700 years of domestication,belong to the complete metamorphosis insects, and it has four states, including egg, larva, pupa and adult. In a long time under the condition of artificial domestication, we get a kind of silkworm which can produce high silk yield through hybrid between two superior quality,to promote the development of C hina’s silk industry. The feed efficiency of high or low influent the silkworm silk production, so improve the feed efficiency is an important content of improving economic efficiency of silkworm.With the rapid development of nanotechnology, promoted the application of nanometer materials in the production and living. Nanometer Titanium dioxide（Titanium dioxide nanoparticles, TiO₂ NPs） as a new nanometer material, due to its many advantages such as small size effect and surface effect, is widely used in medicine, cosmetics, sewage treatment, etc. Because of TiO₂ NPs can promote the production of silk protein, bioactive, and can promote the expression of resistance genes, silkworm have related research reports.In order to study the effects of different concentrations of TiO₂NPs, for the growth and development of silkworm, feed efficiency, and the influence of silk production,promote development and application of TiO₂ NPs as the main raw material in the production of additives, in this paper, "Suju×Minghu" silkworm varieties as research material, add fresh study different concentrations of TiO₂NPs（5 mg/L, 10 mg/L, 20 mg/L, 40 mg/L, 80 mg/L, and 160 mg/L） on the biological effects of the silkworm, the main results are as follows: 1 Effects of TiO₂ NPs on Silkworm Growth and Feed EfficiencyIn order to study the effect of different concentrations of TiO₂ NPs on the biological effects of the silkworm, this experiment investigated the weight change of the silkworm, the quantity of food, digestion and digestibility of the amount. We found that low concentrations of TiO₂ NPs（5 mg/L and 10 mg/L） were more effective for weight gains, with significant weight gain being obtained at 72 h（P < 0.05）. TiO 2 NPs at 20 mg/L or higher had certain inhibitory effects, with signifi-cant inhibition to B. mori growth being observed at 48 h. The feed efficiency was significantly improved at low concentra-tions of 5 mg/L and 10 mg/L for 14.6% and 13.1%, respectively（P < 0.05）. All B. mori fed with TiO₂ NPs showed increased cocoon mass and cocoon shell mass; at 5 mg/L and 10 mg/L TiO₂ NPs, cocoon mass was significantly increased by 8.29 %and 9.39%, respectively（P < 0.05）. We also found that low con-centrations（5 and 10 mg/L） of TiO₂ NPs promoted B. mori growth and development, improved feed efficiency, and in-creased cocoon production, while high concentrations（20 mg/L or higher） of TiO₂ NPs showed inhibitory effect to the B. mori. Consecutive feeding of high concentrations of TiO₂ NPs led to some degrees of adaptability. This study provides a reference for the research on TiO 2 NPs toxicity and the basis for the development of TiO₂ NPs as a feed additive for B. mori. 2 Based on the development of nano-Ti O2 silkworm feed additive effects on the productionapplication effectThis study, the experiment to get the optimal concentration of TiO₂ NPs（5 mg/L） on the production application promotion, statistics the incidence of silkworm, cocoon production. After production application show that the use of additives, silkworm to virus disease, bacterial disease and other silkworm disease resistance are stronger than the control, including "Suju × Minghu" other silkworm disease incidence of 2.16%, the control area is 4.86%, reduce the incidence of 55.56%. "Suju × Minghu" and "Jingsong × Haoyue" experimental zone of ten thousand head of silkworm cocoon quantity than control area is 1.4 kg and 1.5 kg respectively, showed that additive can improve the output of cocoons. Additive processing district, "Suju × Minghu" the whole cocoon weight, cocoon weight and cocoon layer ratio were 1.87 g, 0.44 g and 23.49%, in the cont rol area respectively 1.83 g, 0.4 g and 21.65% additive processing zone "Jingsong × Haoyue" the whole cocoon weight, cocoon weight and cocoon layer ratio 1.88 g, 0.42 g and 22.48%, the control area respectively 1.92 g, 0.46 g and 24.12%. The above data show that the additive can increase the yield and quality of cocoons, with building of mulberry silkworm body at the same time, improve the characteristics of silkworm disease resistance, which laid a foundation for the industrialization application of the additive.