The Change And Excitation Mechanism Of Ultra-weak Luminescence In Postharvest Strawberry Fruit Senescence Under The Reactive Oxygen And Energy Regulation

’HongYan’strawberry fruit were used as the test material in this experiment which regulated by exogenous reactive oxygen species (H2O2), active oxygen scavenger (tea polyphenols), energy inhibitor (DNP) and exogenous adenosine triphosphate (ATP). The change and excitation mechanism of UWL in postharvest strawberry senescence under the reactive oxygen and energy regulation were explored in this experiment. The main results are as follows:1. UWL intensity showed a downward trend in strawberry Fruit aging process. H2O2 and DNP treatments accelerated the senescence of strawberry fruit, and increase the downward trends of UWL intensity; tea polyphenols and ATP can delay senescence process, and slowing UWL downtrend. Which prove that UWL can reflect the degree of aging, because the UWL decline with the strawberry fruit senescence. The aging process and strawberry fruit UWL intensity can be affected by the regulation of reactive oxygen and energy.2. In the process of Strawberry fruit senescence, active oxygen accumulation showed an upward trend, while UWL intensity showed a downward trend. H2O2 and DNP treatments accelerated the accumulation of reactive oxygen species, and increased the UWL intensity decreasing; tea polyphenols and ATP treatment decreased the accumulation of reactive oxygen species, and weaken the decline of UWL intensity. H2O2 treatment increased the accumulation of reactive oxygen species in mitochondrial, and decreased the UWL intensity; tea polyphenols cleared the accumulation of reactive oxygen species in mitochondrial, and increased the UWL intensity. Under the control of active oxygen and energy, UWL intensity in strawberry fruit and mitochondria were negatively correlated with active oxygen, and weakened with the accumulation of active oxygen. These results suggest that UWL indirect effected by reactive oxygen species while reactive oxygen species was not the directly reason of UWL.3. In the process of strawberry fruit senescence, UWL intensity weakened with the energy decline. H2O2 and DNP treatments accelerated energy reduced and increasing the UWL intensity decreased; tea polyphenols and ATP treatments delayed the decrease of the energy and the decline of UWL intensity. DNP treatment reduced energy level and UWL intensity in mitochondrial rapidly; ATP treatment increased mitochondrial energy levels and UWL intensity. Under the control of active oxygen and energy, UWL intensity were positively correlated with energy, UWL intensity was weakened with the decreasing of energy level. The results prove that energy could be a direct source of UWL.