Comparative Transcriptional Analysis Of Rabbit Spleen Infected With C-strain And Shimen-strain

Pig industry occupies a large proportion in the agricultural economy of our country. Classical swine fever (CSF) is one of the important factors affecting the healthy development of the pig industry, so prevention and control of CSF is listed in the National Long-term Animal Disease in China. From the mid-1950s, C strain was used to control the CSFV but not so well. Scholars generally agree that C strain originated in Shimen strain, this attenuated vaccine strain producing strong immunity protection in pigs and it can aslo cause rabbit fever. This study trying to expain the different transcriptional regulation of C strain and Shimen strain induced in rabbit spleen and select for some key genes and pathways in host response. The ultimate purpose of this study is to develope further of the pathogenesis of CSFV and the molecular mechanism of C strain adapting to the rabbits. The main results obtained are as follows:1. Monitoring the rabbit temperature changes post-infectiom C strains and Shimen strain 12h,24h,30h,36h and 48h. Temperature began to"rise at 30h of rabbits infected with C strain and over 1℃ at 36h. There was no significant change of rabbits infected with Shimen strain.2. Cmparative analysis of transcriptional regulation showing both of C strain and Shimen strain can accomplishment proliferation by changing host cell cycle, immune response, inflammatory reaction and so on.3. This two strain stimulated RIG-I like receptor, Toll-like receptor, NOD-like receptor mediated innate immune response in rabbit. Innate immune response is body’s first line of defense against foreign microbes, it can also promote host adaptive immune responses against pathogens. Rabbit fever maybe the production of adaptive immune response.4. There may be special amino acid sequence in the capsid protein of CSFV, its hydrolysis with host antiviral protein reduce the antiviral effects and makes CSFV replication and spread in host easily.5. After infection with C strain, CD 163, LBH, MS4A3, ATG10, S100 (A8, A9, A12), DEFB124 up regulated in rabbit spleen; CCL5, CD36, IL18, LTF, BPI up regulated in rabbit spleen after infection with Shimen strain; CYR61, CD94, DUSP1, IF135 up regulated in rabbit spleen between infection with C strain and Shimen strain. DUSP1, IFI35 up regulated in rabbit spleen between infection with C strain and Shimen strain.