The Responding Mechanism Of Physiology And Proteomics In Prunus Mira Roots Under Drought

Drought stress is one of the major adversity affect plant normal growth, due to its directly affected by rainfall, it is difficult to control, on crop yield or not drought-tolerant plants survival will have a huge impact. Plant roots is the main organ of plants to absorb water and nutrients, it is the part of plants to respond to drought at the first moment. Increase drought resistance of the root system will improve the plant to a reasonable allocation of water, reduce the stress of a series of damage, increase the chances of survival of plant stress under.Prunus mira (3 weeks) was the research object in this study, five different drought water gradient was set as follows:4 days,8 days,12 days,16 days, and re-watering. We study the root proteome and the main physiological changes compare with the corresponding control group.Under drought stress, the growth of Prunus mira main root was restrained as the increased drought degree, lateral root number increased to promote horizontal suction; Root water content gradually reduce, recovered after re-watering; Root malondialdehyde (MDA), hydrogen peroxide, proline content and relative conductivity increased with the increase of drought degree and decreased after re-watering; the activity of root antioxidant enzymes (POD, APX and CAT) increased with the increase of drought degree and decreased after re-watering.Two-dimensional electrophoresis technology was used to study the proteome of Prunus mira root system under different drought degree of differentially expressed, each group about 420 protein points were detected. By comparing the most severe drought stress group (S16) with the re-watering group (S20),95 common protein points in each group were identified as sequencing and database objects. The number of protein points upregulated in S16 and downregulated in S20 was 16, downregulated in S16 and upregulated in S20 was 9, upregulated both in S16 and S20 was 9, downregulated both in SI6 and S20 was 6, upregulated in S16 and no significantly changed in S20 was 23, downregulated in S16 and no significantly changed in S20 was 33. Through NCBInr, interpro, Swiss database, the measured protein can be divided into 12 function classifications:cytoskeleton dynamics, carbon and nitrogen metabolism, energy metabolism, transcription and translation, transport, inducer, stress and defense, molecular chaperone, protein degradation, signal transduction, other materials metabolism and the unknown function protein.Function of stress and defense proteins occupied the largest proportion, most of this part upregulated in S16 and downregulated in S20. In addition, function of transcription and translation protein spots also occupied large proportion as well as. function of molecular chaperone. So Prunus mira root will start art mechanism under drought stress, reduce the damage of root system structure and function. This study explores Prunus mira root art for looking for drought resistance gene to lay the theoretical foundation, improvement of resistance of light walnut and other plants will have good prospects for development.