Study On Fecal Immunoglobulin Level Of The Does And Fawns Of Captive Sika Deer

This work aimed to study the immune state in the does and fawns of captive sika deer (Cervus nippon hortulorum) in lactation period according to the non-invasive sampling method. In Shuangyang Deer Farm, we determined the content of fecal immunoglobulin (IgA, IgM and IgG) of 12 does from 10 to 90 days after delivery, and 12 fawns from 10 to 130 days after born every ten days. For Zuojia Deer Farm, we tested the content of fecal immunoglobulin (IgA, IgM and IgG) of 3 artificial feeding fawns from 20 to 50 days every ten days. In Taiyangdao Deer Park, we determined the content of fecal immunoglobulin (IgA, IgM and IgG) and cortisol of 1 doe from 10 to 90 days after delivery, and 1 fawn from 30 to 90 days after born every ten days. The results are as follows:1. For the natural feeding does, the levels of IgG, IgM and IgA showed an upward trend in 40 days after delivery. And the levels of IgG and IgA decreased significantly (P<0.01), however, the IgM content presented a slight rebound after decreasing, and finally stabilized.2. For the natural feeding fawns, the levels of IgG, IgM and IgA showed an upward trend in 40 days after born. In 50-80 days after born, IgG content were in downward trend, IgM content kept steady relatively, the IgA content showed a sudden decline at 50 days (P<0.01) and tended to be stable. With the levels before weaning, the levels of IgG and IgM after weaning were decreased highly significantly (P<0.01) and IgA level was not significantly decreased after weaning.3. In feces of natural feeding does, IgM showed highly significant and positive correlation with IgA and IgG (P<0.01), and cortisol was highly significant and positive correlation with IgM and IgA (P<0.01). In feces of natural feeding fawns, IgG showed significant and positive correlation with IgM and IgA (P<0.05), and cortisol was significant and positive correlation with IgG, IgA and IgM (P<0.05).4. From 20 to 50 days after born, the levels of IgG, IgM and IgA in feces of artificial feeding fawns were significantly lower than the natural feeding fawns in the same period (P<0.01).5. Sika deer is a kind of social animal, the cortisol content in feces of does and fawns that fed individually showed significantly higher than in flocks (P<0.01). Comparing with the does and fawns fed in flocks, the does and fawns fed individually were under greater pressure.Through the determination of the levels of fecal immunoglobulin (IgA, IgM and IgG) to evaluate immune health status of the does and fawns of sika deer in lactation period and explore the relationship of immunoglobulin between does and fawns deer, and also could provide the basis for scientific deer feeding.