Preliminary Analysis Of Soil Microbial Diversity In Ny-Alesund,Arctic

Soil microbe of Arctic is an important part of polar ecosystem. The community structure of soil microbe can reflect the effects of soil environment and climate changes. In this study, 11 soil samples were collected during the Arctic Yellow River Station scientific investigation in 2015 and 4 four typical soil samples were collected during the Arctic Yellow River Station scientific investigation in 2013. The method of isolation and culture even 454 high-throughput sequencing technology were used to obtain the information of soil microbial community structure respectively. Combined with the soil physical and chemical properties, possible causes were preliminary explored for the structural differences of bacterial community in different typical soils.A total of 74 culturable bacteria and 12 culturable fungi were obtained from the 11 different soils. According to the colonial morphology observation, 65 bacteria and 12 fungi were sequenced. 16 S and ITS sequences were alignmented and phylogenetic analysis. And then, Mega 6.0 software were used to perform phylogenetic tree with the neighbor-joining method. After that, classified information of soil microbe were obtained. In this region, the predominant genera in bacteria are Flavobacterium, Arthrobacter and Pseudomonas and the predominant fungi is Mortierella.Under different types of vegetation, 4 typical soil samples were collected and analyzed by 454 high-throughput sequencing. Statistically analysis were performed after sequencing. The result showed differences in phylum level and obvious differences in genera level. Then a RDA analysis were made combine with the communities of bacteria with the physical and chemical properties to find that the bacterial communities in different soils were affected by different factors, and this finding may cause the differences in genera and OTU composition. The results of analysis show that there is a strong correlation between bacterial community structure and soil physical and chemical properties. Such as SiO₄^2--Si and NH₄⁺-N may have a greater impact on bacterial community structure.