Study Paos Of Different Forms Of Phosphorus Transformation In The Role Of Minerals And Black Soil

Phosphorus is one of the three necessary nutrients for plant growth and development, and it is also a limiting element for water eutrophication. There are mainly two forms of phosphorus exist in the enviroment, inorganic phosphorus and organic phosphorus, but since only water-soluble phosphorus in the soil can be uptaken and utilized by crops, even though the soil has enough total phosphorus, the majority of the world’s agricultural soils present ‘short of phosphorus’. Since there is a positive correlation between the content of soluble phosphorus in soil and crop yields, in the production practice, increasing P and activate soil insoluble phosphorous are two important methods to solve soil phosphorus deficiency, and as the recognition and acceptance of academic point of ‘P crisis’ recent years, exploitation and utilization of soil insoluble phosphorous and phosphorus recycling technique in sewage treatment have become hot area in soil science and environmental engineering studies.Soil phosphorus transformation is biological microbe-based drive process. PAOs widely existed in various types of soils, it is a important group influence water-soluble phosphorus content of soil and soil phosphorus availability and migration. There are los of reports about PAOs in the sewage treatment process, like under aerobic conditions, excess water-soluble phosphorus absorbed by cells and stored in the form of metachromatic granules, while depolymerize under anaerobic conditions and release phosphorus have been widely reported. However, there are little reports about phosphorus forms can be used by PAOs and the role of PAOs in soil phosphorus transformation. The objective of this study was to access the role of PAOs isolated from chernozem in different forms of phosphorus transformation, to enrich the scientific understanding and evaluate role of phosphate accumulating bacteria in soil phosphorus cycle.The results of this study are shown as below:1. Using of toluidine blue selective medium(PAOs medium) isolated 42 PAOs, 6 strains have more than 79% of the phosphorus accumulating rate, one is fungus, theother 5 is bacterias;2. Under the culture conditions in this expeirment, phosphate rock is the sole phosphorus source, roles of the six PAOs in mineral phosphorus transformation was evaluated. The results show that: the ability of mineral phosphorus utilization is different for different PAOs, strains J4-2-5、J4-3-1 can’t use mineral phosphorus; The strain J3-2-1、J2-3-2、J2-1-1、J4-2-7 can use mineral phosphorus, after 24 hours of incubation, the phosphorus content in culture liquid is6.32mg/L 、 5.97mg/L 、 5.81mg/L、5.46 mg/L 、5.35 mg/L、6.58 mg/L; Effect degree of different carbon sources on the role of PAOs in phosphate rock were, in order, glucose, sucrose, starch, cellulose sodium.3. Under the culture conditions of this study, take black soil as tested soil, roles of the 6 PAOs in different forms of phosphorus transformation were evaluated. The results show that: the ability of mineral phosphorus utilization is different for different PAOs; The strains J4-2-5 can’t use mineral phosphorus The strain J3-2-1、J2-1-1、 J4-3-1、J4-2-7、J2-3-2can use mineral phosphorus, after 24 hours of incubation, the phosphorus content in water-soluble phosphorus、Ca2-P、Ca8-P、Al-P、Fe-P、O-P、 Ca10-P content in culture liquid is 5.94mg/kg、4.35 mg/kg、24.59 mg/kg、9.78 mg/kg、 12.34 mg/kg、4.93 mg/kg and 27.32 mg/kg; Different strains with different forms of phosphorus in the order of a water-soluble phosphorus, Ca2-P, Al-P, Fe-P; difficult to use phosphorus Ca8-P, OP and Ca10-P; different strains use of soil in different forms of phosphorus order size J3-2-1, J2-1-1, J4-2-7, J2-3-2, J4-3-1; effect of different carbon sources on the order of PAOs in the black soil of glucose, sucrose, starch,cellulose sodium.